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  1. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ... means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or ... Ashley Waldron...

  2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ... psoriasis Generalized pustular psoriasis is a chronic autoinflammatory skin disease estimated to affect...genetically-encoded biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes...

  4. Working with Nuclear Receptors

    Type
    Blog Post
    ...for endocrine steroids (i.e., corticosteroids, progesterone, androgens, and estrogens), fat-soluble vitamins... the genome to sense the impact of the total environment on the cell. The NURSA Consortium Now in its ...

  5. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ...

  6. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ...solution that has been thawed more than once. Fibronectin is another membrane-interacting protein that ... 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral ...channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use viral prep...

  9. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... fidelity, while the 52-piece assembly of a lac operon dropped to 49% fidelity, with significant lot-to-lot...Assembly, a great place to start is the 24-piece lac operon assembly deposited by NEB – and don’t forget to...

  10. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...sites on the target protein they tend to have a strong signal and to work in a variety of applications...Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283...

  11. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post
    ...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or... Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. doi:10.3389/fpls.2015.01001 Ho...

  12. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post
    ...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping...depend on your blot — antibodies that bind more strongly or that have saturated the blot may need longer...

  13. Tips for Technical Support Calls

    Type
    Blog Post
    ...start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it? Please address as soon ... If the customer service rep does not tell you upfront how long it will take to get an answer, consider...

  14. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...scientists can get involved in modelling, the electronics folks can build a robot worm on top of that model...final target decisions, we intend to focus on motor neuron knockouts so that we can perturb factors related...

  16. Experimenting with New Careers while in Grad School

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    Blog Post
    ...career path. Luckily, in grad school much of the up front work was done for me. I just had to take advantage...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

  17. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post
    ...repair. NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In...plasmid integration; however, since NHEJ is error-prone, such a system is likely to introduce additional...

  18. Of Myc and Men

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    Blog Post
    ...binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all the things! ... Ashley Waldron...

  19. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...What if a tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during... References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence for Encapsidation...
Showing: 401 - 420 of 671 results