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We narrowed to 676 results for: RON

Showing: 401 - 420 of 676 results
  1. Working with Nuclear Receptors

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    Blog Post
    ...for endocrine steroids (i.e., corticosteroids, progesterone, androgens, and estrogens), fat-soluble vitamins... the genome to sense the impact of the total environment on the cell. The NURSA Consortium Now in its ...
  2. 5 Steps to Writing Grant Proposals for Grad Students

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    Blog Post
    ...institution. Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to...will convince the reviewers that the proposal is strong, well-thought out, and will be successful when ...
  3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post
    ...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or... Technology is a Challenge yet Worth Accepting. Front Plant Sci. 2015;6. doi:10.3389/fpls.2015.01001 Ho...
  4. Antibodies 101: Stripping and Reprobing Western Blots

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    Blog Post
    ...isn’t too different, consider antibody affinity: stronger binding antibodies will require harsher stripping...depend on your blot — antibodies that bind more strongly or that have saturated the blot may need longer...
  5. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral ...channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use viral prep...
  6. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post
    ... fidelity, while the 52-piece assembly of a lac operon dropped to 49% fidelity, with significant lot-to-lot...Assembly, a great place to start is the 24-piece lac operon assembly deposited by NEB – and don’t forget to...
  7. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ...sites on the target protein they tend to have a strong signal and to work in a variety of applications...Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods in Cell Biology (pp. 283...
  8. Tips for Technical Support Calls

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    Blog Post
    ...start: “Hi! We ordered something from you and it’s wrong. Can you help us fix it? Please address as soon ... If the customer service rep does not tell you upfront how long it will take to get an answer, consider...
  9. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ...solution that has been thawed more than once. Fibronectin is another membrane-interacting protein that ... 7. Stockschläder, M., et al. "Expansion and fibronectin-enhanced retroviral transduction of primary human...
  10. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ...scientists can get involved in modelling, the electronics folks can build a robot worm on top of that model...final target decisions, we intend to focus on motor neuron knockouts so that we can perturb factors related...
  11. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post
    ...What if a tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during... References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence for Encapsidation...
  12. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post
    ...researchers have realized how problematic aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...
  13. Magnetic Control of Proteins: More than a Dream

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    Blog Post
    ...developing a new fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et al., 2024...science in the open The York Lab operates with a strong commitment to exploration and collaboration. Andrew...
  14. Pooled Library Amplifications

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    Blog Post
    ... library in a lentiviral backbone might be more prone to recombination, while a library with a large backbone...transcription factor overexpression library) or is prone to recombination, we recommend amplification on ...
  15. Of Myc and Men

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    Blog Post
    ...binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all the things! ... Ashley Waldron...
  16. Plasmids 101: Knockout/Knock-In Plasmids

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    Blog Post
    ...the resistance cassette has been placed into an intron.    Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd...
Showing: 401 - 420 of 676 results