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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 472 results for: Sele

Showing: 401 - 420 of 472 results

  1. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...for effective over-agar selection has been empirically determined. See selection curve below. Carbenicillin..., plated over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using...1 mg/mL and 2 mg/mL plates and effective selection. Selection Curve of Transformed E. coli after Over-...methodology for plating antibiotic over-agar for the selection of transformed E. coli . Over-agar spreading of...easy for an investigator to conveniently plate and select transformed cells containing plasmids differing...agar. This protocol will focus specifically on selection with carbenicillin; however it can be adapted ...adapted for any antibiotic through the use of a selection curve to determine optimal antibiotic concentration...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... target selection are continuously being improved. Below are suggestions for target selection. Use an ...concentration F.3 Protocol for lentiviral infection and selection G. Safety H. References H.1 Published articles...encoded in pLKO.1 allows for convenient stable selection. Figure 1 : Map of pLKO.1 containing an shRNA ...puromycin. Puro R Puromycin resistance gene for selection of pLKO.1 plasmid in mammalian cells. sin 3’LTR...replication. Amp R Ampicillin resistance gene for selection of pLKO.1 plasmid in bacterial cells pUC ori pUC...Determining the Optimal 21-mer Targets in your Gene Selection of suitable 21-mer targets in your gene is the...an siRNA selection tool to determine a set of top-scoring targets for your gene. For example, the Whitehead...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...enables selection of a stable cell culture after transduction. Performing antibiotic selection on transduced...This is the beginning of the selection process, which will begin the selection of a stable cell pool. Observe...transducibility of the cell line used, this antibiotic selection may be a vital step for obtaining a population...Remove media, replace with fresh media containing selection reagent Day 3–14: Change media as needed Day 14...Lentivirus preparation Appropriate antibiotic for selection (e.g. puromycin, blasticidin) Reagent Preparation...Before beginning, determine the optimal dose of selective reagent for your target cell line. To do this,...resulting in massive cell death upon antibiotic selection. The number of cells transduced should be enough...

  4. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... thought on using selective medium throughout subsequent experiments. When selective medium is used, it...was used for the initial selection). Addgene recommends that the use of selective medium throughout experiments...site(s) of proviral integration across cells. Selective pressure on this heterogeneous cell pool could...the polyclonal cell pool is always undergoing selection, it’s best to generate monoclonal cell lines from... sure that the cell pool has been sufficiently selected with the appropriate antibiotic so that every ...biological effects on the cell line. In the absence of selective medium, there is a chance that the integrated ... Some researchers choose to maintain cells in selective medium at a reduced antibiotic concentration (...

  7. Depositor Collections

    Type
    Collection
    ...Pleiades Promoter Project: mini-promoters to drive selective expression in the brain - Simpson Reagents for...

  8. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...plants. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene...

  10. CRISPR Plasmids - Parasites

    Type
    Collection
    ... efficiency than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR...

  12. Colony Formation Titering Assay

    Type
    Protocol
    ... of selective reagent for 12 days with media exchanges every 3–4 days. After 12 days of selection, no ...adapted to a variety of target cell lines and selection markers. Note this assay requires staining resistant... 2: Replace media with fresh media containing selection reagent Days 3–14: Change media as needed Days...Colonies were allowed to grow in the presence of selective reagent for 12 days with media exchanges every...

  14. Mammalian RNAi Tools

    Type
    Collection
    ...with BioRender.com. shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids...

  15. Serotype Testing AAV

    Type
    Collection
    ...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

  17. AAV for Neuronal Tracing

    Type
    Collection
    ...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

  18. Retrovirus Plasmids

    Type
    Collection
    ... plasmid) MSCV For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...

  20. Chemogenetics AAV Preps

    Type
    Collection
    ...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...
Showing: 401 - 420 of 472 results