We narrowed to 535 results for: genome
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TypeBlog Post... can be used to integrate the tagged AID* into genomic sites of interests via CRISPR techniques. This ...
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GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...G protein-coupled receptor genes from the human genome sequence. FEBS Letters 520:97–101 . https://doi.org... -
Plasmids 101: FLEx Vectors
TypeBlog Post...on the Addgene Blog Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog Post... Cre. Could Cas9-DOG be developed soon to allow genome modification only in a small subset of cells? Other... -
The Future of Research Symposium Boston 2015
TypeBlog Post...Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research Associate at the... -
Lentivirus Plasmids
TypeCollection...These viruses also integrate stably into the host genome, enabling long term transgene expression. There...because this virus integrates randomly into the host genome, it has the potential for insertional mutagensis...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This ...Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material enclosed in the viral...lentivirus is used for research, it is the lentiviral genome that encodes genetic material that the researcher... wants delivered to specific target cells. This genome is encoded by plasmids called "transfer plasmids... -
CRISPR Plasmids - Bacteria
TypeCollection...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...pLtetO-1 Fujii Efficient isolation of specific genomic regions and identification of associated proteins... -
CRISPR Plasmids - Parasites
TypeCollection...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection... plasmids for CRISPR-Cas and Cascade for large genomic deletions....Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions generated by Cas9... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...multiple components) to target locations in the genome defined by a guide RNA. The transposase can then...insertions. CASTs are most often used in bacteria genome editing, though some mammalian systems have been... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...classifications are still being created as whole-genome sequencing becomes more relevant (Garcillán-Barcia... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...questions around climate change, biodiversity, or genomes requires lots of data. Get your community involved... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post... types of DNA, such as cDNA, PCR fragments, or genomic DNA. Also remember that commercially available ... -
Plasmids 101: Protein Expression
TypeBlog Post...expression of genes integrated into the insect cell genome. Both of these types of insect expression systems... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...induction of T7 polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced... -
Hot Plasmids - October 2020
TypeBlog Post...protein identified exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...carries a small, approximately 4.7 kb single stranded genome. AAV belongs to the family Parvoviridae and requires... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...sequence (Epigenetics literally means ‘on top of the genome’). Epigenetic modifications can result in phenotypic... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...alternatives to BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http://www.ebi.ac.uk... -
Lentiviral Prep Service
TypeCollection...interrogate the genome in a variety of ways. Cas9 proteins can be guided to specific genomic sites by an ...resource page . Cas9 for Editing Genomic Sequences To perform genome edits, Cas9 nuclease is used to generate...