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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 539 results for: genome

Showing: 401 - 420 of 539 results

  1. Browse

    Type
    Collection
    ...Collections Structural Genomics Consortium Plasmids Browse All SGC Plasmids Structural Genomics Consortium Plasmids...Browse plasmids from the Structural Genomics Consortium (SGC) for 3D protein structure studies Plasmid...

  2. Microbiology Resources

    Type
    Collection
    ...bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome Engineering...using CRISPR in your lab, specific CRISPR tools for genome editing in bacteria, and more. Synthetic Biology...range of organisms. Find CRISPR tools for use in genome editing of bacteria. CRISPathBrick Multiplex Plasmid...opens in a new window) JGI Integrated Microbial Genomes and Microbiomes (IMG/M) (Link opens in a new window...Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window) Return...

  3. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Plasmid Collections Structural Genomics Consortium Plasmids Structural Genomics Consortium Plasmids You may...Plasmids from the Structural Genomics Consortium (SCG), which are primarily used for determining protein...like... David Waugh Lab plasmids The Structural Genomics Consortium is a not-for-profit organization that...

  4. Viral Production

    Type
    Collection
    ...sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated from the final...confirm the identity and integrity of the viral genome and the absence of unexpected DNA contaminants....pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] ...Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles within representative...of a lentiviral vector, incubated for 72 h, and genomic DNA is extracted. Primers and probes targeting ...harvesting cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify...

  5. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...exactly match your genomic target? Variation between a given gRNA sequence and your genomic target may reduce...Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...gRNA plasmids designed to target various genes or genomic regions. It is highly recommended that you determine...

  6. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...recombinase systems, how to use them to modify the genome or control gene expression, and find plasmids for...derived from fungi and bacteriophages are powerful genome engineering tools used to precisely manipulate ... payload containing an attB target site into a genomic attP site. So far, their practical application ...site-specific recombinases can be used to modify the genome or control gene expression or skip ahead to browse... Vectors . Flp-IN: A single FRT site within the genome of a host cell line serves as the target location...stable integration of the gene of interest into the genome at the defined FRT site. Multiplexing: Orthogonal... may cause repetitive recombination leading to genomic instability and cell death. It is important to ...

  7. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy in living cells...sequence to direct the dCas9-FP fusion to a specific genomic sequence - potential target locations can include...

  10. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...expression of genes integrated into the insect cell genome. Both of these types of insect expression systems...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ...protein identified exclusively in huge bacteriophage genomes. It’s active in vitro and in human and plant cells...

  17. Lentivirus Plasmids

    Type
    Collection
    ...These viruses also integrate stably into the host genome, enabling long term transgene expression. There...because this virus integrates randomly into the host genome, it has the potential for insertional mutagensis...conjunction with the plasmid that encodes the viral genome (typically called the transfer plasmid ). This ...Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material enclosed in the viral...lentivirus is used for research, it is the lentiviral genome that encodes genetic material that the researcher... wants delivered to specific target cells. This genome is encoded by plasmids called "transfer plasmids...

  18. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ... Publication Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...the nickase to the target site by homology to a genomic DNA locus and encodes a primer binding site and...Cas9 (dCas9) can be used to purify a region of genomic DNA and its associated proteins, RNA, and DNA. ...pLtetO-1 Fujii Efficient isolation of specific genomic regions and identification of associated proteins...

  19. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...loss of function alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair ...Knockout Pooled Library - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with...

  20. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... plasmids for CRISPR-Cas and Cascade for large genomic deletions....Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...into a DNA “shredder” system that can cause large genomic deletions. Compared to deletions generated by Cas9...
Showing: 401 - 420 of 539 results