We narrowed to 542 results for: SHI

1. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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   Blog Post

   ...Charlotte, all of whom are still working on theirpenmanship. Additional resources on the Addgene blog ...

2. 5 Great Apps for Lab Life

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   Blog Post

   ...selenocysteine and pyrrolysine. I have found myself wishing that it had a codon list for each amino acid, but...

3. Resume Writing for Non-academic Science Careers

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   Blog Post

   ... grant application? Are you familiar with the publishing or patent application processes? How about facilitating...

4. Advice for Scientists Starting a Lab

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   Blog Post

   ... labs. Further Reading & Resources EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists...

5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...blue light which revealed a blue light-dependent shift in mCherry fluorescent to the surface of the bead...

6. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...protein (EBFP2) is expressed in an unregulated fashion and thus a marker for transfection. The other (...

7. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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   Blog Post

   ...thanks to our guest blogger Jon Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has...

8. Luminescent Imaging with Nano-lanterns

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   ... plasmids from this paper at Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent...

9. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ... inserted or deleted, can result in an in-frame shift of mCherry and thus expression. As a proof of concept...

10. Antibodies 101: ChIP

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    Blog Post

    ...alongside your input control. Elute the DNA after washing and then incubate with Proteinase K to reverse ...

11. Hot Plasmids - February 2022

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    Blog Post

    ...frankenbodies (Zhao et al. 2019), Tim Stasevich and Hiroshi Kimura’s labs have recently published a set of ...

12. Viral Vectors 101: Viral Applications

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    Blog Post

    ...-Cas9 tools have been engineered to introduce frameshift mutations in genes of interest, turning expression...

13. GPCRs: How Do They Work and How Do We Study Them?

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    ... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

14. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    Blog Post

    ... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove...

15. Developing a cold tolerant E. coli using specialized chaperones

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    ...’s harshest environments: corrosive pH ranges, crushing pressures, near-melting temperatures, tons of ...

16. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post

    ...the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This massive conference attracted...

17. CRISPR 101: Non-Homologous End Joining

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    Blog Post

    ...consequently about a two-thirds chance of causing a frameshift mutation. Of some importance, the deletion can...

18. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    ...guest bloggers! Nikolai Braun grew up outside of Washington D.C. and has always enjoyed exploring the outdoors...

19. Kiran Musunuru on the Newest TALEN Genome-Editing System

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    Blog Post

    ...desired 15 base pair sequence in the genome in rapid fashion. The two remaining plasmids in the kit have the...

20. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...: 30643213. PubMed Central PMCID: PMC6467691. Takahashi, Melissa K., et al. "A low-cost paper-based synthetic...