We narrowed to 569 results for: CIT

1. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   Blog Post

   ...not get packaged in the AAV vector. The cloning capacity between the ITRs is ~4.4 kb. ITRs are inherently...

2. Meet the New Addgene Board Members!

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   Blog Post

   ..., Frank Stegmeier, and Luk Vandenberghe. We’re excited to grow the team and bring on scientists with a...

3. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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   Blog Post

   ...While the VGS process isn’t new to Addgene, we are excited to share the procedures and Python scripts with...

4. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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   Blog Post

   ...copyright owner and will have the opportunity to cite the image to give proper attribution.  We hope ...

5. New and Upcoming Viral Vectors - December 2019

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   Blog Post

   ...accurate monitoring of neurotransmitters and we are excited to offer AAV for the newest GRAB sensors from the...

6. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   Blog Post

   ...micropipette and gloves at a campsite. And it was exciting – one night while trying to process samples at...

7. New and Upcoming Viral Vectors - Spring 2019

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   Blog Post

   ...-WPRE AAV1 with a CAG promoter exhibits high specificity for Purkinje neurons. 105321  AAV1  pGP-AAV-syn-jGCaMP7c...

8. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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   Blog Post

   ...copies of the transgene cassette are introduced, stochasticity will result in a combinatorial expression of...

9. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   Blog Post

   ...P163T, I175Q, C176T and N177F mutations). Its excitation maximum is reported as 603 nm, while the emission...

10. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post

    ...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual...

11. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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    Blog Post

    ...structure and stability from the scaffold as well as specificity towards a HA epitope from the CDRs.  To visualize...

12. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post

    ...less than one week for material deposits. We were excited to deliver the very first ones in person by our...

13. Plasmids 101: Origin of Replication

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    Blog Post

    ... of origins of replication out there so, for simplicity’s sake, we've ignored those used in eukaryotic...

14. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post

    ...feature is advantageous if you’re working with small capacity viral vectors such as AAV. Base removal RNA-guided...

15. Plasmid Cloning by PCR

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    Blog Post

    ...roughly 18-21bp is usually sufficient to give specificity and to also be compatible with a standard PCR...

16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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    Blog Post

    ... Plasmids 101 (2nd Ed.) eBook And now for some exciting news... You can now download the newest version...

17. Mentoring for Scientists: I Have a Mentor, What Now?

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    Blog Post

    ...Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring"    ...

18. Viral Vectors 101: Inverted Terminal Repeats

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    Blog Post

    ...can be used to drive gene expression at varying capacities. AAV vectors containing the AAV2 ITRs can be ...

19. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post

    ... techniques is its ease of use.  “I was really excited when I saw these two papers,” said the University...

20. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post

    ...photon imaging.   Figure 1: 1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V...