We narrowed to 464 results for: LARGE

1. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ...reagents with the scientific community.   (click to enlarge) As we look ahead to the things to come, we wanted...

2. Antibodies 101: ChIP

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   Blog Post

   ...www.wannapik.com/   Even though ChIP requires a larger upfront investment than many other antibody applications...

3. Behind the scenes: Addgene’s new search engine and more

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   Blog Post

   ...Elasticsearch to support the different usage patterns the larger team identified. The basic function of a search...

4. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   ...activate or inhibit neuronal firing. As GPCRs are the largest class of signal transducing receptors in the brain...

5. Working with Nuclear Receptors

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   Blog Post

   ... receptors and their ligands NRs represent the largest family of transcription factors found in metazoans...

6. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   Blog Post

   ...Johnna's New Under The Sun Blog and is part of her larger tutorial series, A Beginner’s Guide to Writing ...

7. Career Coaching for Scientists: Why and Where Do I Find One?

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   Blog Post

   ...Confident and Successful Industry Professionals”. This larger shop might appeal to some although most scientist...

8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ...packaging capacity of 4-5 kb limits the delivery of larger transgenes, such as base editors which have a gene...

9. Giving gRNAs a Facelift - Synthetic and Beyond

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   Blog Post

   ...targeting is led by a ~20 bp gRNA which is part of the larger CRISPR RNA (crRNA). In addition to the 20 bp guide...

10. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    Blog Post

    ... protein and the target antibody works well. In larger cell populations, you can deliver these components...

11. 6 Tips for Grant Writing

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    Blog Post

    ...helpful in assisting me with applications for much larger fellowships. Also, if you were awarded money to...

12. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post

    ...scientists to publish findings that don't fit into larger publications and would otherwise be lost in lab...

13. Viral Vectors 101: Calcium Sensors

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    Blog Post

    ...whereas non-neuronal systems tend to have slower, but larger, calcium changes. The GCaMP6 family of sensors ...

14. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post

    ...follow journals related to your field of research or larger journals that publish about a broad range of topics...

15. Cpf1 Update: Comparison to Cas9 and NgAgo

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    Blog Post

    ... are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9. These nucleases...

16. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...protein sequences (nr)” database as it contains the largest number of protein sequences. Just below the BLAST...

17. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...systems. Baculovirus transfer plasmids have a much larger packaging capacity — up to 38 kb! Generally, insect...

18. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ...probably comes as no surprise that the USA is the largest depositor and requestor of CRISPR plasmids. But...

19. Tips for a 1st time AAV user (by a Rookie AAV user)

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    Blog Post

    ... This helps avoid multiple freeze/thaws of your larger aliquots of virus. AAV titration by qPCR Overview...

20. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    Blog Post

    .... To compensate for this, researchers can plate larger volumes of the outgrowth containing the transformants...