We narrowed to 682 results for: RON

1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...developed FPs optimized for diverse cellular environments. Since colocalization relies upon the detection...Fluorescent Probes. 7. Barbier, Mariette, and F. Heath Damron. "Rainbow Vectors for Broad-Range Bacterial Fluorescence...

2. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...researchers have realized how problematic aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...

3. Of Myc and Men

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   ...binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all the things! ... Ashley Waldron...

4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   ...apart is the way they generate their color: they strongly absorb visible light to give colors in ambient...Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https://doi.org/10.1128...

5. Plasmids 101: Knockout/Knock-In Plasmids

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   ...the resistance cassette has been placed into an intron.    Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd...

6. Experimenting with New Careers while in Grad School

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   ...career path. Luckily, in grad school much of the up front work was done for me. I just had to take advantage...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

7. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...repair. NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In...plasmid integration; however, since NHEJ is error-prone, such a system is likely to introduce additional...

8. Genome-wide Screening Using CRISPR

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   ... target DNA, which, when repaired through error-prone non-homologous end joining (NHEJ), usually results...of multiple gRNAs targeting the same gene can be strong evidence that a particular gene is actually important...

9. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...orders, tracking students and research projects, electronic lab notebooks and protocol management. Though...More Reading: How to manage scientific data: Electronic lab notebooks compared with Google Docs and wikis...

10. Antibodies 101: Producing Recombinant Antibodies

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    ... you have multiple contaminating bands or a few strong ones, consider increasing the number of washes ...Schirrmann T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...

11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...can occur in two different orientations, but is strongly biased towards T-RL, where the R end is proximal...https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda C, Klompe SE, Chen EE, Acree C, Wang HH, Sternberg...

12. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ... of science, particularly public health and environmental science, by the Trump administration are difficulties... climate change, the degradation of the U.S. environment, and a decline in the competitiveness of U.S....

13. Simplify Cloning with in vivo Assembly

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    ...Biology. He is interested in neuronal signal transduction, and uses electron cryo-microscopy to understand...

14. Plasmids 101: Secondary Nanobody Toolbox

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    ...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...

15. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...across most, if not all, industries and work environments. This is why they are called transferable skills...leader,” because you don’t see yourself  standing in front of a crowd and using your charisma to give people...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...homepage. You may have noticed the search field front and center. Let’s type in “crispr” and see what...doesn’t and you find yourself wading through the “wrong” plasmids or coming up with no results at all, let...

17. An Introduction to Adenovirus

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    ...exposure, a lack of immunity, and then finally a synchronized exposure under these conditions once the measures...proteins and downstream cellular events, all recruit strong innate and adaptive immune responses that inactivate...

18. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...metastasized tumor cells, or alzheimers-affected neurons, or [your cell line of interest], is the foundation...nucleotides arranged in a double helix. People are wrong. In reality, cellular DNA is a complex three-dimensional...

19. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...to this question.  We truly work in a unique environment where, in addition to furthering our mission ...). 3. Do something outside of your ordinary environment Team building events like Escape the Room or ...

20. A Career in Grants: How To Become a Grant Professional

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    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models... a particular area, e.g. education, poverty, environment, early childhood development, or community development...