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  1. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...What if a tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck into the prep during... References and resources References Chadeuf G, Ciron C, Moullier P, Salvetti A (2005) Evidence for Encapsidation...

  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...researchers have realized how problematic aggregation-prone tetrameric fluorescent proteins can be. Cytotoxicity...Takeshi, Zhongmei Li, and Michael S. German. "Chronology of islet differentiation revealed by temporal...

  4. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...the resistance cassette has been placed into an intron.    Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd...

  5. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...career path. Luckily, in grad school much of the up front work was done for me. I just had to take advantage...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

  6. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...repair. NHEJ joins the ends of a DSB in an error-prone fashion, with insertions and deletions common. In...plasmid integration; however, since NHEJ is error-prone, such a system is likely to introduce additional...

  7. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ... target DNA, which, when repaired through error-prone non-homologous end joining (NHEJ), usually results...of multiple gRNAs targeting the same gene can be strong evidence that a particular gene is actually important...

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... you have multiple contaminating bands or a few strong ones, consider increasing the number of washes ...Schirrmann T. Expression of recombinant antibodies. Front Immunol. 2013 Jul 29;4:217. doi: 10.3389/fimmu.2013.00217...

  14. An Introduction to Adenovirus

    Type
    Blog Post
    ...exposure, a lack of immunity, and then finally a synchronized exposure under these conditions once the measures...proteins and downstream cellular events, all recruit strong innate and adaptive immune responses that inactivate...

  18. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models... a particular area, e.g. education, poverty, environment, early childhood development, or community development...

  20. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage explains how lentiviral particles are... of this article. Additional Resources: Didier Trono Lab: Lentivectors Toolbox Resources on the Addgene...
Showing: 421 - 440 of 672 results