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We narrowed to 618 results for: Set

Showing: 421 - 440 of 618 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Gersbach Lab multiplexing plasmids: This plasmid set allows you to express 2-4 gRNAs, with four being ...including Standard PolIII-gRNA cassette, Csy4-cleavable cassette, and PTG cassette.    Find gRNA multiplexing...smaller than constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors...aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" ...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Peripheral Infrastructure Vectors and an Extended Set of Plant Parts for the Modular Cloning System.” PLOS... chromosomal integration of larger expression cassettes (Shaw et al., 2023). Those looking to express ...different promoters, terminators, enhancers, and cross-cassette interactions in gene expression, and to generate...

  4. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...AAV-containing Iodixanol solution will flow from the needle set at the 40–60% interface. Make sure that the microcentrifuge...Protocol Video Watch the protocol video below to learn setup and use an iodixanol column gradient for AAV purification...forth a few times to mix the iodixanol that has settled at the bottom of the column. We recommend concentrating...

  5. Western Blot

    Type
    Protocol
    ...follows: Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top Stack to one side and discard the white separator...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ...Place the gel mix in the autoclave and run on a setting that gets the sample to at least 121 ℃ under 20...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...selection. Use an siRNA selection tool to determine a set of top-scoring targets for your gene. For example...help guide your reaction buffer selection when setting up double digests. Digest pLKO.1 TRC-cloning vector...

  9. CRISPR Library Amplification

    Type
    Protocol
    ...2023 Workflow Timeline Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ... early-onset, where symptoms appear between a person’s thirties and mid-sixties, or late-onset, where ...individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It is more likely... results in death within 3-5 years from symptom onset. Disease mechanisms are still poorly understood ...during or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases...cases of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation in...such as the ε4 allele, are a risk factor for late-onset AD. Many more genes have been associated with Alzheimer's...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...SLC1A3 CMV Episodic ataxia Susan Amara 32868 SETD1A SETD1A His T7 Parkinson's Cheryl Arrowsmith 32880 FLAG-MATR3...Parkinsonism, early-onset Roger Reeves 53822 SYNJ1-170_pCSdest SYNJ1 CMV, SP6 Parkinsonism, early-onset Roger Reeves...ALS Catherine Tomasetto 104450 pEGFPC1-hVAP-B KD/MD VAPB GFP, HA CMV ALS Catherine Tomasetto 104465 hnRNPA2...SERPINI1 Halo Dementia Michael Ward 178169 SETX_Halo_C_allele SETX Halo ALS, Ataxia with neuropathy Michael...C6A/C111A SOD1 ALS Nam-Chul Ha 193056 pADC10-SETXhel SETX Flag CMV, p10 ALS, Ataxia with neuropathy Andrew...infantile onset Smita Patel 205056 SUMO-Twinkle 43-684 TWNK His Spinocerebellar ataxia, infantile onset Smita...disease Kivanc Birsoy 218838 3xFlag-eGFP-Flag-SETX SETX Flag, GFP CMV ALS, Ataxia with neuropathy Albert...

  13. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... an exogenous donor cassette, the donor cassette replaces the target site cassette in a double-crossover... sites (often called a "lox-STOP-lox" or "LSL" cassette) placed upstream of a gene of interest will prevent...-layered genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Rolf Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  14. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...the expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369...gRNA module vectors, multiple gRNA expression cassettes can be assembled using Golden Gate cloning or ...polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled into a Cas9 expressing vector. Yang...to rapidly generate multiple sgRNA expression cassettes, which can be assembled into the binary CRISPR...sgRNAs Zebrafish Plasmids for assembly of gRNA cassettes using Golden Gate ligation into Tol2-based pGGDestTol2LC...

  15. Lentivirus Plasmids

    Type
    Collection
    ...Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common and...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk Parijs ...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan Kissler... plasmid with a chimeric 5’LTR. Any expression cassette (promoter and gene of interest) can be cloned ...interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). Find...

  16. CRISPR Plasmids - Tagging

    Type
    Collection
    ...plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins. The ... The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and gRNA...PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To ...cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for... expression vector allows for efficient marker cassette FLP-out. Plasmids can be found associated with...

  17. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...markers. The FP together with the protothropic cassette can be amplified with the following primers: FW... Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces...., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for ...

  18. Adenovirus Plasmids

    Type
    Collection
    ...deletion in E3 and insertion of GFP expression cassette. Bunz 179202 pAd5-B6/7 ∆E3-GFP Adenoviral A block...deletion in E1 and insertion of GFP expression cassette. Bunz 16402 pShuttle Shuttle For production of...Shuttle Modified pShuttle-CMV plasmid with Gateway cassette; See article for additional plasmids Zhang 50957...

  19. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Plant Expression mStayGold 499 510 136 4.8 Monomer pRSETB/mStayGold - Bacterial Expression mBaoJin (mStayGold...mCarmine in pcDNA3 - Mammalian Expression mCarmine in pRSETB - Bacterial Expression mCardinal 604 659 17 5.3...Mammalian Expression pPAGFP-C1 - Mammalian Expression pRSETA-PAGFP - Bacterial Expression pFA6a-PA-GFP-kanMX6...516 581 405 47 30 5.6 6.4 Prone to dimerization pRSETa mEos2 - Bacterial Expression pFA6a-link-yomEos2...

  20. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...plasmids to insert a second guide within a U6-guide cassette. This process is compatible with all pX458 family...
Showing: 421 - 440 of 618 results