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Showing: 421 - 440 of 616 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...Gersbach Lab multiplexing plasmids: This plasmid set allows you to express 2-4 gRNAs, with four being ...including Standard PolIII-gRNA cassette, Csy4-cleavable cassette, and PTG cassette.    Find gRNA multiplexing...smaller than constructs with multiple promoter-gRNA cassettes, making them advantageous for small capacity vectors...aTet-inducible gRNA and a backbone-targeting gRNA cassette for plasmid curing through "self-destruction" ...

  3. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...AAV-containing Iodixanol solution will flow from the needle set at the 40–60% interface. Make sure that the microcentrifuge...Protocol Video Watch the protocol video below to learn setup and use an iodixanol column gradient for AAV purification...forth a few times to mix the iodixanol that has settled at the bottom of the column. We recommend concentrating...

  4. Western Blot

    Type
    Protocol
    ...follows: Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top Stack to one side and discard the white separator...

  6. Pouring LB Agar Plates

    Type
    Protocol
    ...Place the gel mix in the autoclave and run on a setting that gets the sample to at least 121 ℃ under 20...

  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...selection. Use an siRNA selection tool to determine a set of top-scoring targets for your gene. For example...help guide your reaction buffer selection when setting up double digests. Digest pLKO.1 TRC-cloning vector...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ... early-onset, where symptoms appear between a person’s thirties and mid-sixties, or late-onset, where ...individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It is more likely... results in death within 3–5 years from symptom onset. Disease mechanisms are still poorly understood,...during or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases...cases of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation in...such as the ε4 allele, are a risk factor for late-onset AD. Many more genes have been associated with Alzheimer's...Initiative Collection . Use the 3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...SLC1A3 CMV Episodic ataxia Susan Amara 32868 SETD1A SETD1A His T7 Parkinson's Cheryl Arrowsmith 32880 FLAG-MATR3...Parkinsonism, early-onset Roger Reeves 53822 SYNJ1-170_pCSdest SYNJ1 CMV, SP6 Parkinsonism, early-onset Roger Reeves...ALS Catherine Tomasetto 104450 pEGFPC1-hVAP-B KD/MD VAPB GFP, HA CMV ALS Catherine Tomasetto 104465 hnRNPA2...SERPINI1 Halo Dementia Michael Ward 178169 SETX_Halo_C_allele SETX Halo ALS, Ataxia with neuropathy Michael...C6A/C111A SOD1 ALS Nam-Chul Ha 193056 pADC10-SETXhel SETX Flag CMV, p10 ALS, Ataxia with neuropathy Andrew...infantile onset Smita Patel 205056 SUMO-Twinkle 43-684 TWNK His Spinocerebellar ataxia, infantile onset Smita...disease Kivanc Birsoy 218838 3xFlag-eGFP-Flag-SETX SETX Flag, GFP CMV ALS, Ataxia with neuropathy Albert...

  10. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ... an exogenous donor cassette, the donor cassette replaces the target site cassette in a double-crossover... sites (often called a "lox-STOP-lox" or "LSL" cassette) placed upstream of a gene of interest will prevent...-layered genetic control. Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible...Rolf Zeller Use this dual recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...2023 Workflow Timeline Day 1: Transform, recover, set up overnight growth (Estimated time 2-3 hours) Transformation...

  12. Lentivirus Plasmids

    Type
    Collection
    ...Plasmids Envelope Plasmids Resources Lentiviruses, a subset of retroviruses, are some of the most common and...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk Parijs ...shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan Kissler... plasmid with a chimeric 5’LTR. Any expression cassette (promoter and gene of interest) can be cloned ...interest. Can also express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in the 3´-LTR). Find...

  13. CRISPR Plasmids - Tagging

    Type
    Collection
    ...plasmids for CRISPR-based knock-in of EGFP-2A-PuroR cassette to the C-terminus of endogenous proteins. The ... The PITCh donor plasmid with an EGFP-2A-PuroR cassette, flanked by microhomologous sequences and gRNA...PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To ...cells that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for... expression vector allows for efficient marker cassette FLP-out. Plasmids can be found associated with...

  15. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...markers. The FP together with the protothropic cassette can be amplified with the following primers: FW... Sheff, M. A., Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces...., Nielsen, L. K. (2013). Dual gene expression cassette vectors with antibiotic selection markers for ...

  16. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Plant Expression mStayGold 499 510 136 4.8 Monomer pRSETB/mStayGold - Bacterial Expression mBaoJin (mStayGold...mCarmine in pcDNA3 - Mammalian Expression mCarmine in pRSETB - Bacterial Expression mCardinal 604 659 17 5.3...Mammalian Expression pPAGFP-C1 - Mammalian Expression pRSETA-PAGFP - Bacterial Expression pFA6a-PA-GFP-kanMX6...516 581 405 47 30 5.6 6.4 Prone to dimerization pRSETa mEos2 - Bacterial Expression pFA6a-link-yomEos2...

  17. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...fusion shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...plasmids to insert a second guide within a U6-guide cassette. This process is compatible with all pX458 family...

  18. CRISPR Guide

    Type
    Collection
    ..., D., Romanel, A., Demichelis, F., Inga, A., & Cereseto, A. (2018). A highly specific SpCas9 variant is...Protocols in Molecular Biology . 115 . PMID: 27366891 Rousset, F., Cui, L., Siouve, E., Becavin, C., Depardieu... M. W., Yamano, T., Nishimasu, H., Nureki, O., Crosetto, N., & Zhang, F. (2017). Engineered Cpf1 variants...

  19. Guide to Using Pooled Libraries

    Type
    Guide
    ...selection mechanism. You’ll infect two sets of cells and subject one set to selection while the other serves...genome, while others are restricted to certain gene sets. In cDNA libraries, each plasmid contains a unique... be small, if they are designed to cover only a subset of genes, or very large (e.g., the Toronto KnockOut...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...A53T). Target Accelerator Genetic Pathway Reference Set : From Jesse Boehm , William Hahn , Matthew Meyerson...ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E...TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E...enhancers, 3 polyA signals as well as selection cassettes and tools for inducible gene expression. Myristoylated...
Showing: 421 - 440 of 616 results