Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 949 results for: ache

Showing: 421 - 440 of 949 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...which uses a much shorter crRNA. Cas12-RNP-PAGE achieved high editing frequency (nearly 100% in some cases...method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with multiplexed...successful in primates. The authors used a multispecies approach to generate and screen a library of AAV9 variants...best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier and delivering a transgene...

  2. CRISPR in the Clinic

    Type
    Blog Post
    ...These therapies are delivered with an autologous approach, editing patient cells ex vivo and then returning...but especially concerning when using a systemic approach. Regarding effectiveness, if the editing frequency...meaningful way. Delivery, especially for systemic approaches, has its own unique set of obstacles. Thus far... liver. Similarly, for cardiovascular disease approaches targeting cholesterol, these particles were an...

  3. Hot Plasmids - October 2020

    Type
    Blog Post
    ...brain structure and function, with the advent of each tool expanding our ability to study and understand...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression... FP genes into C. elegans? By using a split FP approach. Split-wrmScarlet is a new split red FP that is... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...

  4. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...way, but rather just labels the whole brain. To achieve labeling of only first-order connections to the...cell type specificity of the starter cell can be achieved by using EnvA-pseudotyped RABV, and only expressing...supplied in trans. Monosynaptic RABV spread can be achieved by modifying the wild-type RABV genome, which ...previously thought. The differences in the inputs to each of the two populations could be used to explain ...

  5. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...non-functional fragments. When these two fragments are near each other, they combine forces to form a functional ...complementation assay (PCA). In the Split-BioID method, each BirA* fragment is then fused to one of two bait ...and Z interact, the two BirA* fragments complement each other to form a functional BirA* enzyme. When this.... (2017). Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...

  6. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ... newly synthesized RNA from the transcription machinery. Terminators are found downstream of the gene ...three RNA polymerases (Polymerases I, II, and III), each responsible for transcribing different types of ...termination are understood and it has been noted that each eukaryotic RNA polymerase terminates differently.... PubMed Central PMCID: PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer...

  7. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques ...promoters which will create unique mRNA transcripts for each gene that is expressed, multicistronic vectors simultaneously... that only a single translation event occurs for each mRNA. However, some bicistronic vectors take advantage... some of these peptides, no consensus has been reached on which peptide works best. Likely the choice ...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...the genome. Native Cas9 has two catalytic domains, each of which cleaves one strand of DNA upon binding ...proteins derived from different bacterial species, each of which interacts with a distinct gRNA that allows...expression with truncated gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying...engineering parameters via a library-on-library approach." Nature methods 12.9 (2015): 823-826. PubMed ...

  9. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ... of the post! Speed: Your grant deadline is approaching or your advisor is demanding results. If you’...Cost: Funding is usually limited - what cloning approach should you use if the budget is particularly tight...however, or simply looking for a more streamlined approach, you might want to give Gateway a try. This method...answer, so don’t be afraid to explore multiple approaches to find out which one works best for you. Happy...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...vectors can be made at high titers equal to those achieved with lentivirus but offer an improved safety profile...increases the possibilities when designing screens. Each serotype has its own tropism, or a preference to... you can use smaller volumes of your library for each experiment. Additional considerations One of the...Performing In Vivo CRISPR Screens Using the FITS Approach Additional resources at addgene.org CRISPR pooled...

  11. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...to, I made it a personal goal to ask one question each day (this may be more or less difficult depending...identifies a potential collaborator from the meeting, reach out to them, pointing out that you heard their talk...handles to help you find other scientists attending. Reach out if you’d like to meet someone and set aside ...to set goals for myself like meet ten new people each day. Exchange business cards. When you’re done networking...

  12. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ... the human genome with fluorescent proteins. For each gene, we needed to design guide RNAs, donor templates...gaussian normalized cut-to-insert distance) This gives each guide a score ranging between 0 and 1. The cutting...target. The CFD score ranges between 0 and 100 for each guide, with 100 being the strongest interaction ...but the protein sequence they encode is not. After each mutation, it will check the CFD score. If it is ...

  13. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...Genentech, will not. The point is that each company has a unique approach, and some might suit your needs better...setting—especially if job applications require teaching experience or high numbers of publications, which...into the general careers section of their web site. Each position is for a specific, pre-approved project...

  14. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...Typically, there is a very small number of ASCs in each tissue and once removed from the body; their ability...generating iPSCs iPSCs and ESCs closely resemble each other in many aspects. Like ESCs, iPSCs have a high...this series, we’ll discuss the two widely used approaches of somatic cell conversion: conversion via iPSC...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

  15. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...this later). Chemigenetic sensors take a hybrid approach and utilize both a genetic component as well as...sensing to signal output slightly slower than other approaches. Functionally, the voltage sensitivity of the...level in vitro. In vivo monitoring has also been achieved with these sensors, but further optimization is...cell level as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness...

  16. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ... to linking pieces of DNA that are far away from each other in terms of base pairs, but close together...method is that a new cell line has to be created for each genomic loci of interest. This is cumbersome and...analyzed with CAPTURE 2.0, with two gRNAs targeting each site. They found that DNA sequences targeted by ...to interact with, and how many promoters or genes each enhancer is interacting with. Super enhancers are...

  17. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ... when I first became a manager. Know your people Each person is unique and should be managed accordingly...writing a grant, thinking about the next project, reaching out to other teams, expanding your network…. I...problem, I was able to resolve this issue - with coaching - before it became a major problem. But I also...always the case or you may not feel comfortable reaching out to your manager. This is when having a mentor...

  18. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... instances of focused attention become magical teaching moments. The models function as thinking tools...instructional materials that resulted from our work with teachers, and to create custom models of proteins for researchers...that the resulting physical model is an effective teaching tool. You can read about Jacob’s initial reaction...BioMolecular Modeling web site to check out our hands-on teaching tools that are designed to make the molecular ...

  19. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...described below (See Figure 1).   Figure 1: Each problem with dimerizing fluorescent proteins is ...increasing the probability that two FPs will collide with each other. The consequences can be dramatic. For a membrane...the Endoplasmic Reticulum and live cell imaging approaches. Erik is also an avid long distance runner, gardner...14581454. PubMed Central PMCID: PMC2173526. 2. Zacharias, D.A., et al., Partitioning of lipid-modified ...

  20. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ... screening), or arrayed on a 96 well plate where each well contains a defined prey fusion.  The former...possible to screen libraries of bait and prey against each other at the same time in order to try and access...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...: PMC2965261. 8. Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, ...
Showing: 421 - 440 of 949 results