We narrowed to 602 results for: she
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TypeBlog Post... get your plasmids out there Beyond these 3 big pushes, we try to increase your work’s visibility in a...
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In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post... were lots of fluorescent proteins made from jellyfishes and corals, but all of them fluoresce outside... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...long vesicles have hollow interiors and a protein shell that excludes water but is gas permeable. These ... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation.... -
Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process
TypeBlog Post...sequence information contributed by the depositor, published backbone sequences, and any known sequences for... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin... -
Plasmids 101: What is a plasmid?
TypeBlog Post...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology... -
Year of the Camelid: Antibody Style
TypeBlog Post...antibodies and affinity reagents. But for a quick refresher, some of the advantages of sdAbs over conventional... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ... -
Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene
TypeBlog Post... Just over a month ago I finished up my PhD in Biological and Biomedical Sciences at Harvard University... -
Management for Scientists: Giving Feedback
TypeBlog Post...Addgene Blog to follow the entire series as it is published. For more reading try The Discipline of Teams ... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post...Addgene Blog to follow the entire series as it is published. Good introductory reference books on managing... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...backbones that have been designed, tested, and published by academic scientists. To help you find the vector... -
Advice for Choosing a Research Project
TypeBlog Post...community. In the long term, the research project establishes your professional brand. It offers colleagues... -
Chemogenetics Guide
TypeGuide...for development into chemogenetic tools. Need a refresher on G-protein coupled receptors? Read the GPCRs... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...well-controlled. The lack of site selectivity typically diminishes the apparent affinity for the ligand, especially... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post..., interests and passions is another great way to shed light on what paths to pursue. Spend some time really... -
Plasmids 101: Origin of Replication
TypeBlog Post...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...using sgRNAiBAR. Image from Zhu et al., 2019. Wensheng Wei's lab at Peking University has developed a...