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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 728 results for: STO

Showing: 661 - 690 of 728 results

  3. Chemogenetics Guide

    Type
    Guide
    ...phospholipase C, which releases intracellular calcium stores. There are currently three Gq DREADDs based on ... new window) Chen X, Choo H, Huang X-P, Yang X, Stone O, Roth BL, Jin J (2015). The First Structure–Activity...46. PMID: 25937170 (Link opens in a new window) Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, ... Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell. 28:112-126.e6....

  11. Finding Your Science Policy Path

    Type
    Blog Post
    ...move into science policy. Read broadly about the historical roots of science policy to connect to your why...

  14. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ... then gate the B cell population and create a histogram of CD19-containing cells versus total cell number...

  19. Promoters

    Type
    Guide
    ...general transcription factor proteins and histones can bind. Histones are proteins found in eukaryotic cells...cells that package DNA into nucleosomes. Histone binding prevents the initiation of transcription whereas...

  20. Cloning

    Type
    Guide
    ...their recognition site allows for the creation of custom overhangs, which is not possible with traditional...have utilized this approach to create compatible custom overhangs that can then be efficiently assembled...fragments of DNA that you would like fused along with custom ordered DNA oligos of 60-80 base pairs in length...

  21. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral particles is removed and stored or centrifuged to concentrate. Crude or concentrated... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein...

  22. Plan Your Experiment

    Type
    Guide
    ...using CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer...overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service...paired with restriction enzymes for further customization Usually paired with a sequencing method Can’...

  23. CRISPR Guide

    Type
    Guide
    ...insertions, or frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the... cytosines in a gene’s promoter or by inducing histone acetylation or demethylation. The most common modifiers... green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence microscopy...gene by shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA...

  24. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...transfection, you could use 1 µl of 10 µM oligo stock for each well on 24-well plate, co-deliver into ...

  28. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...capsids for more information. Browse Addgene’s in-stock viral preps . AAV retrograde (AAVrg) Directed evolution...Packaging Services Packaged-on-Request AAV Preps In-Stock AAV Preps Resources on addgene.org AAV Plasmids ...

  30. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...ng/µL stock into 900 µL PBS in a microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into ...multichannel pipette, add 100 µL of TMB Stop Solution directly to the wells to stop the reaction. Measure the absorbance...different incubation options available. Caution Use the Stop Solution in a well ventilated area and avoid breathing...tube and vortex. 0.5 ng/µL : Add 450 µL of 1 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. .... 0.25 ng/µL : Add 450 µL of 0.5 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0.125 ng/...ng/µL : Add 450 µL of 0.25 ng/µL stock into 450 µL PBS in a microfuge tube and vortex. 0 ng/µL : 450 µL...concentrations of HRP yield a greenish solution. Stop the reaction before any wells display a green product...
Showing: 661 - 690 of 728 results