Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 990 results for: IGH@

Showing: 881 - 920 of 990 results

  5. AAV Production in HEK293 Cells

    Type
    Protocol
    ...PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM, low glucose (1 ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...dissolved. Pro-Tip This step is challenging due to the high viscosity of PEG. Stirring under medium heat will...precipitation. Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer the entire sample ... prep . The clarified supernatant can be kept overnight at 4 °C before proceeding with the purification...

  6. Summer Fun at Addgene!

    Type
    Blog Post
    ...finally warming up in Boston and that means summer is right around the corner. Here at Addgene, we’re always...

  7. Our New Antibody Mascot is....

    Type
    Blog Post
    ...winner will be determined by popular vote. That’s right - you get to choose the new name! Additionally, ...

  14. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...Inoculating an Overnight Liquid Culture . After you have bacterial growth, add 500 μL of the overnight culture...bacteria onto an LB agar plate . Grow your bacteria overnight at the appropriate temperature. Growth conditions...page. The next day you will be able to start an overnight culture for plasmid DNA prep the following day...

  16. Editor's Choice, October 2016

    Type
    Blog Post
    ...for detecting genome edits, to discover why you might want to use Reddit to discuss your science and more...

  17. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...interneurons. Take a look to see if these new preps might enhance your research!   Table 1: New ready-to-use...

  21. Fluorescence Titering Assay

    Type
    Protocol
    ...container Inverted fluorescence microscope Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated FBS and 5...each well of the 6-well dish. Incubate the cells overnight. If using freshly collected virus, filter through...If you anticipate that your viral stock will be higher titer, consider additional dilutions. Dilution ...

  24. Immunocytochemistry

    Type
    Protocol
    ...protocol may need to be adjusted to accommodate slight differences between products. Addgene does not ...mg/mL DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution by diluting...DAPI stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile...Pro-Tip The plate can be wrapped in foil to block light. Remove the secondary antibody and dispose of it...

  27. Colony Formation Titering Assay

    Type
    Protocol
    ...controller Hazardous waste container Reagents DMEM high glucose, Corning 10-013-CV L-alanyl-L-glutamine ...alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat inactivated FBS and 5...colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers...dilutions, and single colonies are visible in the higher dilutions. Last reviewed on: August 31, 2023...

  28. Reflections and Looking Ahead

    Type
    Blog Post
    ...contributions to key research papers are already inspiring higher standards in antibody use (Ayoubi et al., 2024;...

  30. Pipetting Protocol

    Type
    Protocol
    ...to begin using the pipette. To the right is a photo of how you might hold a pipette. Without moving past... P1000 pipette shows the volume adjustment ring right below the plunger. This ring changes the pipette...P20, P100, P200, and P1000 as shown from left to right. Pipette Dispense Volume P2 0.2 to 2 µL P10 1 to...

  32. Science as Art

    Type
    Blog Post
    ...microscope, you too can become an artist. Scientists moonlighting as artists can compete in the Nikon Small World...

  34. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...purified AAV. Option #3 Puncture the QuickSeal tube slightly below the 60–40% interface with an 18 ga needle... (1999): 973-985 (Link opens in a new window) . Right panel: cartoon indicating the position of the needle...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers... viral vectors," (Link opens in a new window) copyright (2006). Figure 3: Example of an SDS-PAGE gel of...
Showing: 881 - 920 of 990 results