We narrowed to 1,006 results for: RING

1. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   ...would need to be generalizable and capable of measuring a variety of molecular signatures or cancer phenotypes...

2. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   ...Cpf1 vs. Cas9, see the table below. Table 1. Comparing the Cas9 and Cpf1 CRISPR Nucleases   Cas9 Cpf1...

3. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   ...of NHEJ to generate translational reading frame-altering indels for “functional knockout” of a gene of ...

4. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   ...2016, we launched our Viral Service and began delivering ready-to-use lentivirus and adeno-associated ...

5. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   ...double-checking details, or just taking a moment to relax. During really stressful days, I would even take advantage...

6. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   ... alternative antimicrobial.  Conjugates And HRP brings us handily to the final topic of this post, the...

7. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   ...materials in our catalog are very well-characterized. During this time, we’ve also created a variety of educational...

8. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   ...audience your primary focus When writing a paper or preparing a presentation, it's easy to forget that you have...

9. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   ... Addgene, we now use next-generation sequencing during our QC process. This way, we can confirm the sequence...

10. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    ...? CasPEDIA operates like many search engines, featuring both a general search bar tool and an advanced...

11. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    ...reproducibility. The Trimmer Lab found that in addition to recovering RNA from hybridomas grown in tissue culture, ...

12. Improved Plasmid Maps Powered by SnapGene

    Type

    Blog Post

    ...for you to find the plasmids you need. We'll be monitoring and improving the maps further after this initial...

13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    ...presented on this at the ENCODE Consortium in Cold Spring Harbor in March. A number of groups were excited...

14. Chemogenetics Guide

    Type

    Guide

    ...through the Gαq/11 G-protein and activate neuronal firing through stimulating phospholipase C, which releases...muscarinic G αq CNO* Increase Ca 2+ Neuronal burst firing Armbruster et al., 2007 rM3D Rat M3 muscarinic ...adrenergic G αs CNO* Increase cAMP Neuronal burst firing Guettier et al., 2009 hM4Di hM2Di Human M4 muscarinic...and Sternson SM (2011). Chemical and genetic engineering of selective ion channel-ligand interactions....Moore CI, Shaner NC, Hochgeschwender U (2024). Engineering luminopsins with improved coupling efficiencies...Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,...

15. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    ...operon, which is responsible for plasmid maintenance during cell division. ccdB codes for the toxic protein...

16. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    ...antibodies to bind to a primary antibody without interfering with the hypervariable region, where an antibody...

17. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    ...transferable skills while working in the lab and by offering advice on areas where you might need some help...

18. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    ...protein degradation Article contributed by Mary Gearing Listen to the TRIM-Away podcast segment CRISPR...

19. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    ...shouldn’t work for free.” Susan also suggests volunteering to write for a non-profit, like she did for ...

20. RNA Extraction Without A Kit

    Type

    Blog Post

    ...further streamline this process and/or stabilize RNA during tissue collection and homogenization. The most ...

21. Developing Lab Management Software for Biology

    Type

    Blog Post

    ...simple task in the lab, but the software should be mirroring the lab procedures, not artificially constraining...

22. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    ... Mary Gearing...

23. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    ...participation in this panel, Professor Stephan will be delivering the keynote address at the symposium. Eve Marder...

24. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    ...showing. Are those experiments the best way of figuring out what’s going on? Is the data of sufficient...

25. 5 Steps to Writing Grant Proposals for Grad Students

    Type

    Blog Post

    ... your work, your advisor may want to mentor you during the grant application process. When my PhD advisor...

26. Lentiviral Vector Guide

    Type

    Guide

    ...lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and the gamma-retrovirus MMLV...LEDGF (also known as PSIP1 or p75 ), a lentiviral tethering gene that helps facilitate integration. Despite...markers, such as the puromycin resistance gene, conferring antibiotic resistance to infected host cells....target. Lentiviral vectors are a common way of delivering these gRNAs, due to their stable expression and...libraries popular for lineage tracing experiments and monitoring the dynamics of heterogeneous tumors. Browse ...References Cronin, J., Zhang, X., & Reiser, J. (2005). Altering the Tropism of Lentiviral Vectors through Pseudotyping...

27. High Complexity Golden Gate Assembly with NEB’s DAD

    Type

    Blog Post

    ...correctly assembled phage genomes, it required a very stringent selection process and benefited from a longer ...

28. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    ...plasmid from being cut and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and...

29. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    ...scientists will contact you if any issues arise during the QC process. As the depositing scientist, you...

30. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    ...the protein-antigen complexes will be washed away during the protocol. The amount of antibody that binds...

31. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    ...week. This is where our new process will begin. Partnering with seqWell, we are using the plexWell technology...

32. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    ...Upon the application of blue light, Vvd dimerizes bringing the C-terminal and N-terminal Cre fragments together...

33. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type

    Blog Post

    ...interactions to remain stable (or to be stabilized) during cell lysis and lysate fractionation. Important ...

34. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type

    Blog Post

    ...Graduates with Degrees in Science, Technology, Engineering and Mathematics, Washington, DC: US Government...

35. Hot Plasmids - October 2020

    Type

    Blog Post

    ...proteins with FingRs By Jason Nasse Since the pioneering days of Golgi and Cajal, neuroscientists have...

36. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    ...Wormbase. And scientists have been continuously sharing their worm strains through the Caenorhabditis Genetics...

37. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    ...Rho-independent terminators, which include naturally occurring terminators, such as T7 and rrnB, as well as engineered...

38. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    ...type-specificin vivoscreening platform. bioRxiv (Cold Spring Harbor Laboratory). https://doi.org/10.1101/2023.06.13.544831...

39. Antibodies 101: Flow Compensation

    Type

    Blog Post

    ... you can always change your compensation values during the analysis portion of your experiment. So, as...

40. Plasmids 101: Degron Tags

    Type

    Blog Post

    ...kDa). This system also requires less genetic engineering than some of the other systems reviewed here....