We narrowed to 497 results for: RAP

1. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   ...blue). Mismatch positions are indicated below each graph. Figure from Kleinstiver et al., 2016. Cpf1’...

2. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   ...grants professional The Grants Profession is in a rapid growth phase. As a result, there are more opportunities...

3. Plasmids 101: Protein tags

   Type

   Blog Post

   ...proteins can be purified using metal-affinity chromatography, usually using a Ni2+ column. Like other affinity...

4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

   Type

   Blog Post

   ...examples. We also developed the inducible translocation trap (ITT) system to analyze signal-induced nuclear translocation...

5. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   ...particular cell type or tissues; this is useful in gene therapy applications where a distinct subset of the cellular...

6. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   ...Janelia Research Campus on the GENIE website.   Bibliography  Ersing, I., Rego, M., Wang, C., Zhang, Y., ...

7. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   ...Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation...

8. Career Insights: Technical Support Specialist

   Type

   Blog Post

   ...developments in the fields of gene and cellular therapies.     Additional Resources on the Addgene Blog ...

9. Antibodies 101: Reading a Flow Plot

   Type

   Blog Post

   ...: While the plot generated often looks like the graph on the left, it might make more sense once you realize...

10. Plasmids 101: Codon usage bias

    Type

    Blog Post

    ... of a protein should necessarily be translated rapidly to produce a protein that functions properly. You...

11. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    ...cutting-edge imaging technologies such as array tomography, super-resolution STORM imaging, and electron...

12. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ...distance runner, gardener, cook, and outdoor photographer.  Additional resources on the Addgene blog ...

13. Lab to Office Culture Shock

    Type

    Blog Post

    ...to Tony from legal, Diana in finance, and Wu in graphic design. You could dread these interactions, but...

14. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    ... by binding the GyrA subunit of DNA gyrase and trapping it in the cleaved complex. This results in DNA...

15. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ...are investigating how the production of cancer therapeutic drugs, vinblastine and vincristine, are regulated...

16. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    ...compatible or not.  Using plasmid incompatibility for therapeutic advantage Although it may seem like plasmid incompatibility...

17. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    ...bacteria in favorable conditions double their numbers rapidly. The knowledge the Singletons gained from spending...

18. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    ...that the clarifications lead to the story being scrapped or broken up into smaller stories. Since this ...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ...  Wang D, Zhang F, Gao G (2020) CRISPR-Based Therapeutic Genome Editing: Strategies and In Vivo Delivery...

20. Hot Plasmids - August 2020

    Type

    Blog Post

    ...by one-step immobilized metal ion adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow...