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We narrowed to 682 results for: RON

Showing: 441 - 460 of 682 results

  1. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...Addgene Depositor (and lentiviral expert) Didier Trono's lab webpage explains how lentiviral particles are... of this article. Additional Resources: Didier Trono Lab: Lentivectors Toolbox Resources on the Addgene...

  2. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...scientists with the ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has...

  3. Hot Plasmids - August 2020

    Type
    Blog Post
    ...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...

  5. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...the chance of the insert being “dropped” in the wrong place, while increasing the number of potential ...

  6. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...Laboratory Golden Gate Cloning videos made by the Nicola Patron group, one of the developers of the MoClo system...extracting and sequencing the plasmid, or both! We strongly encourage you to sequence the entire insert region...

  7. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Epitope tag antibody collection grows By Ashley Waldron We are excited to highlight the Anti-V5 tag antibody...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs...

  8. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...bacterium picking up resistance genes from it’s environment (from DNA left behind after another bacterium...survival. Existing virtually ubiquitously in the environment, there is an intense amount of competition between...

  10. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent ...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  13. Lab to Office Culture Shock

    Type
    Blog Post
    ...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training... until I was out of the lab and in an office environment. Ultimately, it will likely be these skills that...

  15. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    ...system could be as simple as a piece of paper on the front of the incubator containing the passage number, ...ensure the results are reliable. With this two-pronged approach, the many mistakes of the past can be ...

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389...

  20. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...common promoters are U6, H1, and 7SK. These are strong promoters with no tissue specificity, so in some...biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org/10.3389/fendo...
Showing: 441 - 460 of 682 results