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Showing: 441 - 460 of 672 results

  1. Hot Plasmids: Fall 2024

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    Blog Post
    ...Epitope tag antibody collection grows By Ashley Waldron We are excited to highlight the Anti-V5 tag antibody...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs...

  2. Tips for Writing a Good Cover Letter

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    Blog Post
    ...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...

  4. Lab to Office Culture Shock

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    Blog Post
    ...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training... until I was out of the lab and in an office environment. Ultimately, it will likely be these skills that...

  5. To Codon Optimize or Not: That is the Question

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    Blog Post
    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...scientists with the ability to selectively silence neurons in vivo (Slimko and Lester, 2003).  Addgene has...

  6. Hot Plasmids - August 2020

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    Blog Post
    ...- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over conventional antibodies...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...

  9. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...the chance of the insert being “dropped” in the wrong place, while increasing the number of potential ...

  10. Transferable Skills Guide: Career Planning Resources

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    Blog Post
    ...school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent ...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...

  11. Antibiotic Resistance: An Old Solution but a New Problem

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    Blog Post
    ...bacterium picking up resistance genes from it’s environment (from DNA left behind after another bacterium...survival. Existing virtually ubiquitously in the environment, there is an intense amount of competition between...

  13. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...

  14. The Challenges of Cell Culture

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    Blog Post
    ...system could be as simple as a piece of paper on the front of the incubator containing the passage number, ...ensure the results are reliable. With this two-pronged approach, the many mistakes of the past can be ...

  16. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post
    ... Many cell types can be found in the tumor microenvironment (tumor cells, immune cells, blood cells and...recorded events) based on their expected size. The strong signals close to the graph’s origin mainly stem...

  18. Hot Biosensors 2022: Year-End Roundup

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    Blog Post
    ...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...
Showing: 441 - 460 of 672 results