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We narrowed to 676 results for: RON

Showing: 441 - 460 of 676 results
  1. Lab to Office Culture Shock

    Type
    Blog Post
    ...it’s like to be properly onboarded. Most work environments outside of academia provide a formal training... until I was out of the lab and in an office environment. Ultimately, it will likely be these skills that...
  2. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...Laboratory Golden Gate Cloning videos made by the Nicola Patron group, one of the developers of the MoClo system...extracting and sequencing the plasmid, or both! We strongly encourage you to sequence the entire insert region...
  3. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Epitope tag antibody collection grows By Ashley Waldron We are excited to highlight the Anti-V5 tag antibody...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs...
  4. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...the chance of the insert being “dropped” in the wrong place, while increasing the number of potential ...
  5. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...reproducible assay. That information can make you a much stronger candidate!  Additional uses The cover letter is...job with a lot of file management that asks for strong organizational skills. You might think, “I’d be...
  6. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...school and beyond is to live in your lab, right? Wrong! While it is clearly a good idea to be diligent ...them for work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great...
  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...bacterium picking up resistance genes from it’s environment (from DNA left behind after another bacterium...survival. Existing virtually ubiquitously in the environment, there is an intense amount of competition between...
  8. Tips for CRISPR Gene Editing in Mice

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    Blog Post
    ...however, if the microinjection was done as the pronuclei were fusing, you may have several mosaic mice....Daisuke, et al. "Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9...
  9. The Challenges of Cell Culture

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    Blog Post
    ...system could be as simple as a piece of paper on the front of the incubator containing the passage number, ...ensure the results are reliable. With this two-pronged approach, the many mistakes of the past can be ...
  10. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ...compared to one pot saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template...Chaput, J. C. (2010). “Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis...
  11. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...common promoters are U6, H1, and 7SK. These are strong promoters with no tissue specificity, so in some...biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology, 9. https://doi.org/10.3389/fendo...
  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...MTRIA sensors will be useful for investigating neuronal signaling and for engineering improved sensors...Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:880107. doi: 10.3389...
  13. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...
  14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... Many cell types can be found in the tumor microenvironment (tumor cells, immune cells, blood cells and...recorded events) based on their expected size. The strong signals close to the graph’s origin mainly stem...
Showing: 441 - 460 of 676 results