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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 612 results for: Set

Showing: 441 - 460 of 612 results

  1. Retrovirus Plasmids

    Type
    Collection
    ...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...

  3. TALEN Plasmids and Kits

    Type
    Collection
    ... 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment of double-...

  4. COVID-19 Resources

    Type
    Collection
    ...COVID-19 Research Registry COVID-19 Open Research Dataset (CORD-19) LitCovid (NCBI/PubMed) Protocols Protocols...

  5. Rett Syndrome

    Type
    Collection
    ... and 18 months of age. A defining feature, with onset typically between 18 to 30 months, is regression...

  7. Caltech Systemic Capsids

    Type
    Collection
    ...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:...

  10. Guide to Using Pooled Libraries

    Type
    Guide
    ...selection mechanism. You’ll infect two sets of cells and subject one set to selection while the other serves...genome, while others are restricted to certain gene sets. In cDNA libraries, each plasmid contains a unique... be small, if they are designed to cover only a subset of genes, or very large (e.g. the Toronto KnockOut...

  11. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...vasculature (adult marmosets), biased toward astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys... including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets, rhesus macaques Unknown... screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets (Chuapoco et...transduction levels in marmoset brain, with both neurons and astrocytes targeted. While marmoset liver transduction...AAV.CAP-B22 CNS: neurons and astrocytes Mice, marmosets Unknown Goertsen, Flytzanis, et al., 2022 ... of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et al, 2021 Goertsen, Flytzanis...

  12. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...side of a stop cassette, upstream of an effector gene. In cells where the stop cassette is removed during...just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population...cells will express the effector or retain the stop cassette following recombination. Image from Isaacman-Beck... genetic tool that allows for the labeling of a subset of cells with the same genetic makeup, overcomes...effector is expressed. In cells where the stop cassette remains, the effector is not expressed and the...attP sites to drive expression of either a stop cassette or an effector gene. HDVR (2X Hepatitis Delta ... Truncating the attP site upstream of the stop cassette results in decreased efficiency of recombination...

  13. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... satellite colonies? Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...decorative" title="Check icon" %} Just bacteria {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...Excellent No Bacteria and eukaryotes (G418) {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...purpose="decorative" title="Check icon" %} {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...Check icon" %} Carbenicillin Excellent {% icon icon_set="fontawesome-5.14.0" name="Check" style="SOLID...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... switch conditional knock-in cassette. As shown in Figure 2, the cassette should contain a reporter DNA...For this FLEx switch to successfully work, the cassette would need to contain the BFP coding sequence ...antisense orientation (Figure 1, top). The entire DNA cassette would be flanked by two pairs of target sites-...in the opposing orientation, you have created a cassette that will express GeneR only in the presence of...recombination event would excise the reporter from the cassette, allowing for expression of GeneR. This allows...

  15. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...To overcome this obstacle, a positive selection cassette can be incorporated, and used during the differentiation..., but defective for integration [4], the donor cassette is removed leaving behind no accessory transposon...recombination and Footprint-Free™ removal of the selection cassette by excision-only piggyBac™. Image courtesy of ...recombinase technology (Cre/loxP) to remove the donor cassette. While this method can be effective, a loxP site..., it is possible to make the ITR and selection cassette disrupt the target site for the nuclease thereby...preventing re-cutting. Transposase removal of the cassette later restores the target sequence. This allows...

  16. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...resource for spatial biology enthusiasts. This dataset currently includes 1049 community-validated reagents...into conversations with other users. The entire dataset can be downloaded from Zenodo, including the reagent...table or by downloading directly from the Zenodo dataset.     Figure 2: Screenshot showing reagent...HuBMAP) antibody metadata standards. And the dataset is rich. Experimental data includes reagent type...protocols, panels, publications, software, and datasets. It’s also a great place for anyone who has used...

  17. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ... Biological General Repository for Interaction Datasets (BioGRID) in order to increase the accessibility...accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and ...and ready access to stably archived large-scale datasets by computational biologists. Figure 1: ...other species. By accurately compiling genome-wide datasets, which are often obscured in large supplementary...but also allows cross-interrogation of different datasets that might otherwise be hard to identify. ...

  18. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...bacterial selection cassette for growth and cloning in bacteria and a mammalian selection cassette to screen for...cells, you will not need a mammalian selection cassette (e.g. 1435 pSG5L Flag HA). You can also upgrade...with an integrating vector expressing a selection cassette with 5′ and 3′ flanking sequences matching the...fusion proteins. Pick your promoter, selection cassette and visualization vehicle to conduct your custom...

  19. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Producing yeast expression cassettes Traditionally, generating expression cassettes for yeast transformation...-fidelity polymerase) is the ability to remove, reset and ligate mismatched overhangs, yielding scarless...Cloning sgRNAs Production of Yeast Expression Cassettes Generating constructs with multiple point mutations...regions of homology and inserted into a linearized cassette, ready for transformation into yeast that same...

  20. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...resistance cassette and you aren’t introducing an additional antibiotic resistance cassette along with...plasmid backbone contains a kanamycin resistance cassette, you would plate the cells on kanamycin-containing...carrying plasmids that contain a kanamycin resistance cassette will grow. However, often times, the plasmid backbone...
Showing: 441 - 460 of 612 results