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TypeGuide...selection mechanism. You’ll infect two sets of cells and subject one set to selection while the other serves...genome, while others are restricted to certain gene sets. In cDNA libraries, each plasmid contains a unique... be small, if they are designed to cover only a subset of genes, or very large (e.g. the Toronto KnockOut...
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CRISPR Guide
TypeCollection..., D., Romanel, A., Demichelis, F., Inga, A., & Cereseto, A. (2018). A highly specific SpCas9 variant is...Protocols in Molecular Biology . 115 . PMID: 27366891 Rousset, F., Cui, L., Siouve, E., Becavin, C., Depardieu... M. W., Yamano, T., Nishimasu, H., Nureki, O., Crosetto, N., & Zhang, F. (2017). Engineered Cpf1 variants... -
Modular Cloning Guide
TypeGuide...plasmids from different combinations of a common set of components (Weber et al., 2011). This method, ...tools, and more. The MoClo system consists of three sets of cloning vectors (Level 0, 1, and 2) which can...Bacterial Expression Thorsten Mascher , Georg Fritz Two sets of plasmids to generate constructs for Lactiplantibacillus...create plant expression vectors containing several cassettes and generate multi-construct transgenic plants...protein complexes in various hosts. Expression cassettes for individual subunits are first assembled by... -
Immunology Research Plasmids and Resources
TypeCollection...A53T). Target Accelerator Genetic Pathway Reference Set : From Jesse Boehm , William Hahn , Matthew Meyerson...ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E...TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E...enhancers, 3 polyA signals as well as selection cassettes and tools for inducible gene expression. Myristoylated... -
iPSC Neurodegenerative Disease Initiative Plasmid Collection
TypeCollection...cellular background. All cell lines and phenotypic datasets that result from this effort will be broadly shared... -
Zinc Finger Consortium: Nuclease Expression Vectors
TypeCollection...mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and “-” heterodimeric... -
Institute for Protein Innovation
TypeCollection... function, providing a reliable and accessible toolset for scientists worldwide. Figure courtesy of IPI... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection... following intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F... -
COVID-19 Resources
TypeCollection... opens in a new window) COVID-19 Open Research Dataset (CORD-19) (Link opens in a new window) LitCovid... -
TALEN Plasmids and Kits
TypeCollection... 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment of double-... -
Rett Syndrome
TypeCollection... and 18 months of age. A defining feature, with onset typically between 18 to 30 months, is regression... -
Empty Backbones - Choosing Your Perfect Plasmid Backbone
TypeCollection...create stable cell lines in which the expression cassette of interest is incorporated into the host... -
Caltech Systemic Capsids
TypeCollection...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:... -
Molecular Biology Reference
TypeGuide...from DNA. The Genetic Code The genetic code is a set of rules for translating the information encoded ...replicated easily and efficiently in a laboratory setting. Are stable — Plasmids are stable long-term, either...Genetic Code The genetic code can be defined as a set of rules for translating the information encoded ... -
Science Guides
TypeGuide...genome, while others are restricted to certain gene sets. Read More Viral Vectors Find educational guides... -
Gamma-Retroviral Vector Guide
TypeGuide... vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors have...safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus genome. Created...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain... -
Fluorescent Protein Guide: Biosensors
TypeCollection...Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins... -
Lentiviral Vector Guide
TypeGuide... vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have many...lentiviral vectors are also popular in clinical settings for the delivery of gene therapies. Lentiviral...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...vasculature (adult marmosets), biased toward astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys... including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets, rhesus macaques Unknown... screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets (Chuapoco et...transduction levels in marmoset brain, with both neurons and astrocytes targeted. While marmoset liver transduction...AAV.CAP-B22 CNS: neurons and astrocytes Mice, marmosets Unknown Goertsen, Flytzanis, et al., 2022 ... of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et al, 2021 Goertsen, Flytzanis... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...side of a stop cassette, upstream of an effector gene. In cells where the stop cassette is removed during...just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population...cells will express the effector or retain the stop cassette following recombination. Image from Isaacman-Beck... genetic tool that allows for the labeling of a subset of cells with the same genetic makeup, overcomes...effector is expressed. In cells where the stop cassette remains, the effector is not expressed and the...attP sites to drive expression of either a stop cassette or an effector gene. HDVR (2X Hepatitis Delta ... Truncating the attP site upstream of the stop cassette results in decreased efficiency of recombination...