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Showing: 441 - 460 of 618 results
  1. Guide to Using Pooled Libraries

    Type
    Guide
    ...selection mechanism. You’ll infect two sets of cells and subject one set to selection while the other serves...genome, while others are restricted to certain gene sets. In cDNA libraries, each plasmid contains a unique... be small, if they are designed to cover only a subset of genes, or very large (e.g. the Toronto KnockOut...
  2. CRISPR Guide

    Type
    Collection
    ..., D., Romanel, A., Demichelis, F., Inga, A., & Cereseto, A. (2018). A highly specific SpCas9 variant is...Protocols in Molecular Biology . 115 . PMID: 27366891 Rousset, F., Cui, L., Siouve, E., Becavin, C., Depardieu... M. W., Yamano, T., Nishimasu, H., Nureki, O., Crosetto, N., & Zhang, F. (2017). Engineered Cpf1 variants...
  3. Modular Cloning Guide

    Type
    Guide
    ...plasmids from different combinations of a common set of components (Weber et al., 2011). This method, ...tools, and more. The MoClo system consists of three sets of cloning vectors (Level 0, 1, and 2) which can...Bacterial Expression Thorsten Mascher , Georg Fritz Two sets of plasmids to generate constructs for Lactiplantibacillus...create plant expression vectors containing several cassettes and generate multi-construct transgenic plants...protein complexes in various hosts. Expression cassettes for individual subunits are first assembled by...
  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...A53T). Target Accelerator Genetic Pathway Reference Set : From Jesse Boehm , William Hahn , Matthew Meyerson...ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) ABC17, ABCB2, APT1, D6S114E...TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) ABC18, ABCB3, APT2, D6S217E...enhancers, 3 polyA signals as well as selection cassettes and tools for inducible gene expression. Myristoylated...
  5. Institute for Protein Innovation

    Type
    Collection
    ... function, providing a reliable and accessible toolset for scientists worldwide. Figure courtesy of IPI...
  6. COVID-19 Resources

    Type
    Collection
    ... opens in a new window) COVID-19 Open Research Dataset (CORD-19) (Link opens in a new window) LitCovid...
  7. TALEN Plasmids and Kits

    Type
    Collection
    ... 21179091), as well as (v) distinct selection cassettes on pTAL7a and pTAL7b for enrichment of double-...
  8. Rett Syndrome

    Type
    Collection
    ... and 18 months of age. A defining feature, with onset typically between 18 to 30 months, is regression...
  9. Caltech Systemic Capsids

    Type
    Collection
    ...-biased: Neurons - AAV.CAP-Mac, AAV9-X1.1 For marmosets: Broad tropism - AAV.CAP-B22 Cell type-biased:...
  10. Molecular Biology Reference

    Type
    Guide
    ...from DNA. The Genetic Code The genetic code is a set of rules for translating the information encoded ...replicated easily and efficiently in a laboratory setting. Are stable — Plasmids are stable long-term, either...Genetic Code The genetic code can be defined as a set of rules for translating the information encoded ...
  11. Science Guides

    Type
    Guide
    ...genome, while others are restricted to certain gene sets. Read More Viral Vectors Find educational guides...
  12. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors have...safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus genome. Created...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...
  13. Lentiviral Vector Guide

    Type
    Guide
    ... vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have many...lentiviral vectors are also popular in clinical settings for the delivery of gene therapies. Lentiviral...DNA. However, if the plasmid contains a Gateway cassette containing the ccdB gene, a ccdB-resistant strain...
  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...vasculature (adult marmosets), biased toward astrocytes (adult rhesus macaque) Rats, marmosets, green monkeys... including ENS (adult marmosets, infant rhesus macaques) Mice, rats, marmosets, rhesus macaques Unknown... screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets (Chuapoco et...transduction levels in marmoset brain, with both neurons and astrocytes targeted. While marmoset liver transduction...AAV.CAP-B22 CNS: neurons and astrocytes Mice, marmosets Unknown Goertsen, Flytzanis, et al., 2022 ... of inhibitory and excitatory neurons Mice, marmosets Unknown Brown et al, 2021 Goertsen, Flytzanis...
  15. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...side of a stop cassette, upstream of an effector gene. In cells where the stop cassette is removed during...just a fraction of a single genetically-defined subset of cells. By labeling fewer cells in a population...cells will express the effector or retain the stop cassette following recombination. Image from Isaacman-Beck... genetic tool that allows for the labeling of a subset of cells with the same genetic makeup, overcomes...effector is expressed. In cells where the stop cassette remains, the effector is not expressed and the...attP sites to drive expression of either a stop cassette or an effector gene. HDVR (2X Hepatitis Delta ... Truncating the attP site upstream of the stop cassette results in decreased efficiency of recombination...
Showing: 441 - 460 of 618 results