We narrowed to 542 results for: SHI

1. Hot Plasmids - October 2020

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   ...available as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...

2. A Look at Addgene's QC Process

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   ...the plasmid. This includes point mutations, frame shifts, missing elements, and nucleotide indels or mismatches...

3. Antibodies 101: Stripping and Reprobing Western Blots

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   ...Note that βME reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time...

4. Plasmids 101: Modular Cloning

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   ...identify the best cocktail of genes when the relationship between genotype and phenotype is best described...

5. Viral Vectors 101: Calcium Sensors

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   ...Goltstein PM, Portugues R, Griesbeck O. Putting a finishing touch on GECIs. Frontiers in molecular neuroscience...

6. Viral Vectors 101: Chemogenetics

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   ...BL, Jin J (2015) The First Structure–Activity Relationship Studies for Designer Receptors Exclusively Activated...

7. Viral Vectors 101: Virus Safety

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   ...not currently provided. Eye protection or face shields are required whenever there’s a splash or spray...

8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ...Multiple FAST Fluorescence-activating and absorption shifting tag (FAST) for use with green-yellow or orange-red...

9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ... dCas9-TET, allowing one to study the causal relationship between DNA demethylation and gene expression...

10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...Sathyan V, Nguyenla X, Van Dis E, Catamura C, Yamashiro LH,  Begeman A, Stark JC, Shon DJ, Fox DM, Puschnik...

11. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...the Hirsh family, we are able to take greater ownership of this process with in-house sequencing! In addition...

12. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    ...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...

13. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    .... Since makerspaces are generally funded on a membership model - everyone contributes each month - with...

14. How-to: 5 Steps to a Great Panel Discussion

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    ...attention of the audience – phones come out, postures shift, they are checking out. If you can, plant 1-2 good...

15. 3D Printing Meets CRISPR Cas9

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    ...potential of this protein to undergo conformational shifts. This backbone model of Cas9 is based on Fuguo ...

16. 10 Basic tips for mammalian cell culture

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    ... incubator. This also means that any medium or washing buffer, such as PBS (Phosphate Buffered Saline)...

17. Common Injection Routes in Mice

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    ...131–141. https://doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. ...

18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    ...any prime editor experimentalist, and especially shines once large-scale prime editing experiments come...

19. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...emerged as a particularly useful tool for those wishing to run larger panels: five laser spectral flow ...

20. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...grow the bacterial strain in which the plasmid is shipped. Copy number (if known), antibiotic resistance,...