We narrowed to 732 results for: SON

1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

   Type

   Blog Post

   ...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ...

2. Plasmids 101: Plasmid Incompatibility

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   Blog Post

   ...plasmid. Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of ...

3. An Inside Look into Addgene's Dev Team

   Type

   Blog Post

   ... area, and when something urgent comes up, that person is the first in line to resolve it for expediency...

4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ...the ability to cleave the RNA target.  Table 1: Comparison on Common CRISPR Enzymes Name  Enzymatic Domains...

5. Using Video to Share Your Science: We Share Science

   Type

   Blog Post

   ...me. I read a variety of psychology, business, personnel, and sociology journals when I get a chance, but...

6. Deciphering the Mysteries of Behavior with Viral Vectors

   Type

   Blog Post

   ...Central PMCID: PMC6333924. Howerton, Alexis R., Alison V. Roland, and Tracy L. Bale. "Dorsal raphe neuroinflammation...

7. Designing Your Chalk Talk for the Academic Job Interview

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   Blog Post

   ...compatible functional livers.” Why are you the best person to do this research? Do you have innovative tools...

8. QC Sequencing Technologies at Addgene

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   Blog Post

   .../10.1021/ac2010857  Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating...

9. CRISPR 101: Any Base Transversion Editors

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   Blog Post

   ... Zhu, Y., Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion editors...

10. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ... Rachel Leeson...

11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type

    Blog Post

    ... 1. Atasoy, D., Y. Aponte, H. H. Su, and S. M. Sternson. 2008. “A FLEX Switch Targets Channelrhodopsin...

12. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    ...getting outside academia. There is also a valuable lesson for the enterprise: the time for talk is over and...

13. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...trans-splicing or overlapping vectors: a quantitative comparison . Molecular Therapy, 4 (4), 383–391. https://...

14. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    ...sequence of the protein of interest. One of the reasons that this works well is that the N- and C-terminus...

15. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    ...-based solutions or open source solutions. In comparison to paper-based record keeping, ELNs offer functionalities...

16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    ... the cells used in a given experiment. For this reason, the AAV-based approach requires G418 selection...

17. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    ...Cox Cammer, N., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and optogenetics of...

18. Filming Science Videos in the Age of Social Distancing

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    Blog Post

    ... outside at a distance, or film inside with one person in a room. I wouldn’t be in there with them. I ...

19. Five Popular Model Organisms, Part 2

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    Blog Post

    ...research (Ellenbroek and Youn, 2016). For these reasons rats were once the most used mammalian model in...

20. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    ..., Kosik, K. S., Arias, C., Acosta-Alvear, D., & Wilson, M. Z. (2020). A Scalable, Easy-to-Deploy, Protocol...

21. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    ...0.5% correctly edited alleles, when screening reasonable amounts of single cell clones. Furthermore, CRISPR...

22. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ...Harbor Protocols 2011.1 (2011): pdb-prot5546. Richardson, Alexander, et al. "Visualizing the Fate of Transplanted...

23. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...we’ve previously seen in the CRISPR field, the lessons learned in pursuing one technological application...

24. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ...Future of Research (FOR) Boston Symposium 2015. I personally participated as a member of the Career Paths ...

25. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    ...designs and we need to be transparent about the reasons we alter these designs if we do so. The most extreme...

26. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ...function. The best tools are only as good as the person using them, and the proper use of CRISPR technology...

27. CRISPR 101: Off-Target Effects

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    Blog Post

    ...sequence/whatever your editing event outcome is at a reasonable number, the likelihood of an off-target confounding...

28. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    ... M. A., Hoeppner, L. H., Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., ...

29. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    ... by Mary Gearing in 2018, and updated by Hannah Dotson in Dec. 2022.  References and Resources References...

30. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    Blog Post

    ...GRSMs, we recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts...