...Their work involves coordination and can require technical mastery. Scientists need to be doers for a long...

... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

...expression pattern of a defined promoter region, ectopically expressing YGOI in a pattern that allows the ...

...Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including only...

...AAV viral preps to easily find and share useful technical data. These data focus on experimental design ...

...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting...

...guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than the RNA guides employed by...

...benefits to researchers conducting basic or early pre-clinical research. However, standard terms and techniques...

...Roth’s lab are already available allowing you to chemically control neuronal activity while studying your...

...created by James Trimmer, PhD, of the University of California Davis School of Medicine. This deposit includes...

...coronavirus and SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet...

...Although the sequence and regulation of the ori dramatically affect the copy number of a plasmid, other external...

... that will bring about the next revolution in biological research. We’re incredibly happy to see that ...

...positions posted on recruitment websites, this job specifically looked for a researcher with 3 years+ of cancer...

...incredibly useful, yet simple system allows you to systematically study gene function with temporal control and...

... or pink. Purpose Gram staining can be used in medical settings to determine the general type of bacteria...

...ensures that everyone gets a chance to talk and less vocal team members still have their voices heard. Here's...

...Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / 561 / 640 nm). In our hands the best...

...cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme...

...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...

...site. A specificity ratio can be measured by calculating the on-target activity divided by the off-target...

... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...

... and forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants...

...in target gene knockdown. Note: this plasmid was called pLKO-Tet-On in the original publication but its...

...on Twitter: @ThePurplePage and @HelloPhD "Role" call. #BLACKandSTEM what do you do? — Stephani Page, ...

...effects of inactivating/activating mutations. In biomedical research, modeling patient mutations using SDM...

...Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing!  A recent...

... disciplinary communities gather at ComSciCon's local and specialized franchise workshops throughout the...

...information is organized in wiki format and includes biochemical properties, previously established uses, and ...

... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...

...of an open-access recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The...

...m always catching up in the morning! 9:00 AM--Technical implementation review. The developers discuss ...

...from the Neuroscience Information Framework (NIF) called the Resource Identification Initiative. During ...

...With our protocol, it is feasible to produce genetically modified cells in less than a month's time. Addgene...

...EF1alpha, CBh) or inducible (Tet-ON); U6 promoter is typically used for gRNA May contain reporter gene (e.g. ...needs to match the target locus, but it is also critical to ensure that the gRNA target sequence does NOT...target with no homology elsewhere in the genome. Realistically, a given gRNA target sequence will have partial...additional sites throughout the genome. These sites are called off-targets and should be examined during gRNA ...

...dosage in E. coli using DIAL strains." Journal of biological engineering 5.1 (2011): 10. PubMed PMID: 21787416...

...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...

...too long in the incubator. The small colonies are called satellite colonies and they form when all the antibiotic...

... targeting the Fc region of an antibody, the so-called ‘constant’ region which is, quite usefully, both...

... the desired level of antibodies is reached - typically within 2-4 months. After the animal has the desired...