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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 682 results for: RON

Showing: 461 - 480 of 682 results

  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...light-sensitive ion channels used to control activities like neuronal signaling — think of a mouse with an LED brain... — but there’s a lot more to optogenetics than neuronal activity. Researchers have designed a variety ...

  2. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... Many cell types can be found in the tumor microenvironment (tumor cells, immune cells, blood cells and...recorded events) based on their expected size. The strong signals close to the graph’s origin mainly stem...

  5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...compared to one pot saturation mutagenesis. Error-prone PCR: This method amplifies the wild-type template...Chaput, J. C. (2010). “Random Mutagenesis by Error-Prone PCR”. Methods in Molecular Biology In Vitro Mutagenesis...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the repository, we...Genome-Editing Tool: Application in Improvement of Crops. Front Plant Sci 7: . https://doi.org/10.3389/fpls.2016.00506...

  7. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...Joanne's Reddit AMA 6. Practice, practice, practice in front of a real audience Good, even great, speaking skills...help you avoid giving only yes or no answers in front of a crowd. Having answer slides ready after your...

  8. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...restore the dystrophin reading frame. gRNAs bind in intronic regions, so low-frequency indels will not cause...exonic regions comes with much more risk than the intronic editing used in the exon skipping approaches here...

  9. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...excessive costs in your lab. That in itself is a strong negotiating tactic, and puts you ahead of most ... create productive and manageable laboratory environments. His business skills enhance his passion for...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...mediated dsDNA breaks: following a cut, the error-prone nature of non-homologous end joining (NHEJ) often...optimized sequence will do little if it is in the wrong place, but because the target window is more-narrow...

  11. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...reputation? If you are choosing a training lab, I strongly suggest you complete a work rotation in the lab...and non-academic career paths. I can’t say this strongly enough - don’t choose a mentor that sees you only...

  13. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...brought together and microinjected into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or...remember that you are working with RNAs, which are prone to degradation by the omnipresent RNase. You may...

  14. Finding Your Science Policy Path

    Type
    Blog Post
    ...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and everything...policy training programs and fellowships have a strong focus on early career researchers who have decided...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ...extreme sequence divergence that requires a multi-pronged analysis to sort effectively. The current classification... functions within Class 1 CRISPR-CAS systems. Frontiers in Microbiology, 12. https://doi.org/10.3389/fmicb...

  16. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...10.1021/acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR...

  17. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability, or other....1021/ar200099f  Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., & Johnsson, K. (2003...
Showing: 461 - 480 of 682 results