We narrowed to 767 results for: SCR

... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...

... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...

... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...and can immediately undergo replication and transcription. However, with scAAV the effective genome size...

...the opsin into the correct orientation to be transcribed (Figure 2) (Sharma and Zhu, 2014). How does FLEx...simultaneously recorded the electrical impulses of discrete subpopulations of this brain area, demonstrating...

.... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9...

...blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons function... et al. Cell. 2021) Conduct multimodal genetic screens in human iPSC-derived neurons with a CRISPRi-based...plasmids expressing SOX10, OLIG2, and NKX6.2 transcription factors for rapid and efficient generation of...astrocytes to dopaminergic neurons using three transcription factors in a single polycistronic lentiviral...

...performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / ...photoswitching. In 2008, scientists used mutagenesis screens to find a version of Dronpa that doesn't become...

... tested and will help healthcare professional prescribe and identify potential beneficiaries. Collaborative...Collection Pages Plasmid Grant to Aid Cervical Cancer Screening in Ghana Modulate Cancer Signalling Resources...

...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas...

...the CGBE architecture. They conducted a CRISPRi screen to identify endogenous genes that impact CGBE efficiency...•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning....

... on Request for many more. Metal Ions Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded...Yan Qin Return to top Small Molecules Target Description Article PI Acetyl-CoA PancACe acetyl-CoA sensor...Frommer Return to top Neurotransmitters Target Description Article PI Acetylcholine Family of ACh sensors...Clapham Return to top Cell Physiology Target Description Article PI Autophagy Measure autophagic flux ...Near-infrared fluorescent voltage indicator nirButterfly Screening and Cellular Characterization of Genetically Encoded...caspase", "mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible for Viral Packaging...

...KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles in processes ...elements or GC-rich regions on DNA to mediate transcriptional activation and/or repression. Continuing collaborative...Addgene's collection of KLF plasmids. ID Plasmid Description Gene/Insert PI...

...CRISPR plasmids for transcriptional repression of target genes....Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. This interference is most efficient when the gRNA...

...frequently mutated gene in human cancer, is a transcription factor and tumor suppressor. Wnt Coming soon... Labs containing wild-type and mutant alleles described in the initial Target Accelerator publications... Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer...

...for shuttle plasmids with inserts. ID Plasmid Description PI 16402 pShuttle For production of viruses containing...adenoviral vector production. ID Plasmid / Kit Description PI 16400 pAdEasy-1 Adenoviral vector for use ... for adenoviral vector production. ID Strain Description PI 16398 BJ5183 E. coli strain for recombination...

...CoV-2), modification, or variant. ID Plasmid Description Mutations Industry PI Due to export control regulations...neutralizing activity are listed below: ID Plasmid Description PI 44965 NL4-3 mCherry Luciferase Lentiviral ...provirus and the overall strength of viral transcriptional response in these cells. Warner Greene 105621...

...Plasmid Gene/Insert PI Kits Kit name Type PI Description High Complexity Golden Gate Assembly Standards...Pooled Libraries Pooled Library name Type PI Description HR700_TP53 Exon Mutation Libraries CRISPR Thorsten...exon 5–8. Phagemid Synuclein VHH Immune Library Screening Messer and MJFF Created for research in Parkinson's...

... packaging eligibility, and more. ID Plasmid Description PI Return to top AAV Packaging Plasmids Packaging...for all available serotypes. ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid,...repository for AAV vector production. ID Plasmid Description PI Return to top Other Resources Addgene's Viral...

...below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 230681...labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. Jonathan Ting Viviana Gradinaru...

...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors By Species...