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We narrowed to 958 results for: Tes

Showing: 481 - 500 of 958 results

  1. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Vectors via Different Routes. Diabetes, 55(4), 875–884. https://doi.org/10.2337/diabetes.55.04.06.db05-0927...delivery by directed evolution of underexplored sites on the AAV capsid. Molecular Therapy. Methods & ...

  2. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...(Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16, 2014, that... it has been repurposed for genome editing in eukaryotes, with the most widely used CRISPR genome editing...specifies the target, the Cas9 endonuclease that creates the DNA double-strand break (DSB) at the target...mouse zygote (Qin et al., 2015).  When inside the zygotes, the gRNA will seek out its target among the 3 ...

  3. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...country of origin with an international mentor creates a closer and more approachable relationship for...evidence of this, you needn’t look any further than testimonials from the students who participated in the program... of Oviedo, the European University Miguel de Cervantes, the University of Navarra, and the University...macular degeneration. She is also a blogger who writes posts for the blogs of MasScience, ECUSA, IMFAHE...

  4. Optogenetics Guide

    Type
    Guide
    ...References Introduction The field of optogenetics integrates optics and genetic engineering to measure and...photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent amplitude...msec (hChR2) to “long lasting,” e.g. seconds to minutes with stable step-function opsins (SSFOs). Opsin...flavin mononucleotide as its chromophore, and phosphorylates the transcriptional activator FixJ in the absence...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...the surface of extracellular pathogens such as parasites or microbes, or to proteins expressed on the surface...cascades that clear these infections. Anything that generates an antibody response in the immune system is referred...at different times of the immune response and initiates different immune cascades. In the research setting...quantitative measurement of protein expression in cell lysates, whole cells, or tissue samples. Many experiments...

  6. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ... announce the winners of our 2021 Deck the Lab Contest! Though 2021 had more than its fair share of challenges... challenges, the annual Deck the Lab Contest brought us smiles and joy. In fact, our Addgenies were absolutely...festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https...

  7. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ... start looking to find one. Luckily, there are websites that can help: Antibody data repositories, which... Website Targets Application Purpose Notes Antibodypedia Any Any Data repository ...Molecules group Addgene Data Hub Any (see notes) Any Data repository Only accepts data for...

  8. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...through 2009, though I suspect that our costume contests were indeed occurring earlier but that the photo...protect the innocent revelers. Our Halloween costume contests have become an annual tradition in which we choose...finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout our FB page on...

  9. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...our in-stock service: ddPCR, purity testing, and sterility testing, plus viral genomic sequencing to confirm...AAVrg and 7 × 1012 GC/mL for AAV2. There are no quotes or special processes involved; simply request your...

  10. New Viral Vectors - March 2024

    Type
    Blog Post
    ...only March!)  In our repository, you'll find the latest iteration of GCaMP, GCaMP8, available in new serotypes...repository  Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New...forgot to use the link below to keep up with the latest in Addgene's viral vectors services and resources...

  12. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...in the United States but around the world. As an organization that directly collaborates with NIH-funded... are constrained. This collaborative approach eliminates redundant work, saving time and allowing precious...

  14. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...to activate gene transcription. The researchers tested a variety of combinations to optimize both fusion...500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft of Cas9, the fluorescent...Upon light activation, dCas9 is released within minutes and red foci formed 20-40 min later. The CASANOVA...synthetase, read this article). The group first tested photocaging various lysines in Cas9 to determine...

  15. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...environmental impact of those processes? Who even regulates them? You may be surprised to learn that it hasn... been 50 years since the creation of the United States Environmental Protection Agency, which started ...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...

  16. Reflections and Looking Ahead

    Type
    Blog Post
    ...enriched our repository with 15,020 new plasmids — a testament to the scientific community's commitment to open...brings us closer to making an impact on humanity's greatest challenges. Thank you for being part of this incredible...future of science. Want to learn more about our latest developments? Subscribe to the Addgene newsletter...

  17. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...So, as 2013 comes to a close, let’s take a few minutes to reflect on some of the most noteworthy developments...database to gather the results of Myriad's genetic tests for breast and ovarian cancer risk directly from...granted via a public repository or authors’ own web sites. 9)      The year 2013 has also been the year of...

  18. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ... was pumpkin. Everyone gets to vote for their favorites, and trophies are awarded and coveted. I’ve only...we head into Thanksgiving weekend in the United States, I look forward to gathering around a large table...you have any traditions that you share with your labmates, be they food related or not? We would love to...

  19. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ..., M.W., Nguyen, T.H., Coxe, M.A., Miller, B.C., Yates, K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., ..., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of exhausted CD8+ T cell subpopulations...0480-4 LaFleur, M. W., Nguyen, T. H., Coxe, M. A., Yates, K. B., Trombley, J. D., Weiss, S. A., Brown, F....

  20. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...transcriptional regulators. In this system, Cas9 generates a double-stranded DNA break at the genomic loci... the universal sgRNA is not predicted to target sites in the human, pig, or zebrafish genome, this system... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...
Showing: 481 - 500 of 958 results