We narrowed to 783 results for: post

1. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...empty backbones.   Our Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's...

2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ...This post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-...

3. Five Popular Model Organisms

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   ...the effects of genetic manipulations. In this blog post, we’ll cover five popular model organisms, but there...

4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ... proteins. For more details, check out this blog post from the researchers who developed Tetbow. Conclusion...

5. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...engineering other enzymes. Conclusion We hope this post has illuminated some of the many tools available...

6. Plasmids 101: Expressing shRNAs for RNA Interference

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   ...Promoter Region – Let's Go! Browse All Plasmids 101 Posts Additional resources on addgene.org Mammalian ...

7. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   ...briefly recapitulate what we’ve covered in this blog post. First, you learned about the types of scattering...

8. Hot Biosensors 2022: Year-End Roundup

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   ...and SGZ). Read more from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness...

9. Evolution of Lab Techniques

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   ...This post was contributed by guest blogger, Krissy Lyon, a PhD candidate in Neuroscience at Harvard University...

10. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post

    ...This post was contributed by guest blogger, Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute...

11. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ...oversized cargo (Hirsch et al., 2010). Today’s blog post will give brief overviews of three split vector ...

12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...like Addgene (a good example is our series of blog posts on the defunct genome editing technology NgAgo)....Harvard University) Tony Cijsouw (Neuroscience postdoc at Tufts University) Scientific reproducibility...

13. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    ... improved variations you could try. We hope this post helped you start planning for your next multiplex...

14. Hot Plasmids: Spring 2025

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    Blog Post

    ...Plasmids articles. Here's what you'll find in this post: Glutamate indicators with increased sensitivity...synaptic event and they are rapidly taken up by postsynaptic receptors and transporters. The intensity-based...

15. Hot Plasmids: Summer 2024

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    Blog Post

    ...Plasmids articles. Here's what you'll find in this post: MagIC-Cryo-EM for enrichment and structure determination...

16. PiggyBac-ing Through the Genome Editing Field

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    Blog Post

    ...technology from the folks at Transposagen. This post was contributed by Amar Singh and Mathew Goodwin...

17. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ...due to differences in the expression of receptors, post-translational modifications, and other factors that...

18. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ... This post was contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum...

19. Viral Vectors 101: Optogenetic Tools

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    Blog Post

    ...them to be successful in your experiments? In this post, we will discuss some broad classes of optogenetic...

20. Viral Vectors 101: AAV Variables That Matter

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    Blog Post

    ...affect your AAV experiments. Luckily, the other posts in our Viral Vectors 101 series can help guide you...axonal transport  (Murlidharan et al., 2014), and postsynaptic transmission (Oh et al., 2020). For example,...Others, like AAV1 and AAV9, can also transduce postsynaptic cells when used at very high titers (Zingg et...

21. Antibodies 101: Introduction to Antibodies

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    Blog Post

    ...on our Antibodies 101: Polyclonal Antibodies blog post. Monoclonal antibodies On the other hand, monoclonal...

22. Sequencing Options for CRISPR Genotyping

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    Blog Post

    ...This post was contributed by guest blogger Søren Hough, a Biochemistry PhD candidate at the University...

23. CRISPR Guide

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    Guide

    ...screens, see our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries...

24. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ...where they can scan for any signs of danger. In this post we’ll focus on how fluorescent proteins can and ...

25. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

    ...This post was contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University...

26. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

    ...surprisingly easy. It’s true, I CRISPRed. In this post, I’ll summarize the basic steps and analyses, and...

27. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

    ...This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs...

28. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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    Blog Post

    ...for your experiment more complicated. We hope this post helps you in your search for the perfect way to ...

29. How to Deposit Your Plasmids with Addgene

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    Blog Post

    ...What's more, depositing is free! We've written this post as a step-by-step guide to the online deposit process...

30. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ... This post was contributed by guest blogger James D. Fessenden, an Assistant Professor at Brigham and...