We narrowed to 532 results for: abo.3

... restraining further replication.   Figure 3: Comparison of replication regulation mechanisms of... negative regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single...copy number and compensating for the additional metabolic cost imposed on the host with a beneficial function.... On the other hand, they also impose a lower metabolic cost on cells and produce lower amounts of protein...

...biotechnology 16(7): 657–62. PubMed PMID: 9661200.  3. Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking... on the Addgene Blog Learn More about FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs...expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and...

...activation. Second-generation plasmids include (Figure 3): Transfer plasmid — contains transgene and wild-type... expression from the LTR is Tat-dependent. Figure 3: Second-generation lentiviral plasmids. Created with... transfer plasmids also contain a deletion in the 3’ LTR to make them self-inactivating (SIN). This deletion...requirement for Tat. Always includes deletion in the 3' LTR for self-inactivation LTR Viral Promoter Wildtype...) plasmids. These plasmids have a deletion in the 3' LTR of the viral genome that is transferred into .... The inside out of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID...Molecular Therapy — Methods & Clinical Development , 3 , 16017. https://doi.org/10.1038/mtm.2016.17 PMID:...

...visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine deaminase base ...for base-editing activity The reporters mentioned above all rely on GFP fluorescence and cell sorting using... Additional resources on the Addgene blog Read about other CRISPR reporters Browse our CRISPR blog posts...

...types can be found on Addgene’s website. Fig. 3: Cas9 repressors include dCas9 alone and a dCas9-KRAB... 101 Series Learn about the PAM Requirements of Different Cas9 variants Learn about CRISPR Pooled Library...

...Biotechniques 35.4 (2003). 812-4. Pubmed PMID: 14579747. 3. Drexler, H.G., Uphoff, C.C. “Mycoplasma contamination...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...Get Tips for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide...

..." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, Benjamin J., et al. "High-throughput droplet... Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

...concentration of approximately 0.2-0.5 μg/mL (usually about 2-3 μl of lab stock solution per 100 mL gel). EtBr...different buffers and do not use water). Microwave for 1-3 min until the agarose is completely dissolved (but...cool down to about 50 °C (about when you can comfortably keep your hand on the flask), about 5 mins. Optional... of the tip of the pipette into the buffer just above the well. Very slowly and steadily, push the sample... of the way down the gel. A typical run time is about 1-1.5 hours, depending on the gel concentration ...

... interest into fragments containing precise 5' or 3' single-strand overhangs (sticky ends), or no overhang...Gateway attB1 and attB2 sites attached to the 5’ and 3’ ends of the DNA sequence. This fragment can then ...which naturally leaves a single A overhang on the 3' end of PCR products. The complementary T comes from...covalently bound to the phosphate group on the free 3' T. The topoisomerase acts as a ligase that joins ... Read more in our TOPO Cloning blog post . Figure 3: Summary of TOPO cloning. TOPO = topoisomerase I. ... Ligation Independent Cloning (LIC) relies on the 3' to 5' exonuclease activity of T4 DNA polymerase. ...Learn about different molecular cloning techniques, focusing on seven common cloning methods. Educational...

...enables additional flexibility in pseudotyping. Figure 3: Gamma-retroviral vector packaging methods. Created...self-inactivating (SIN). These plasmids have a deletion in the 3' LTR of the viral genome that is transferred into ...vectors: Biology, technology and application. Viruses , 3 (6), 677–713. https://doi.org/10.3390/v3060677 PMID...found on each side of the provirus. The U3 (unique 3’) contains sequences necessary for activation of viral...transcription. R is the repeat region. U3 in cis Unique 3’; contains sequences necessary for activation of viral... RNA transcription. Removal of this region in the 3’ LTR creates self-inactivating viral vectors. R in... with a constitutive promoter such as CMV or RSV. 3' LTR in cis Terminates transcription started by 5'...

...Reference Rq(R165L) Human M3 muscarinic Arrestin-2/-3 CNO* Increase Arrestin translocation Arrestin signalling...5HT3 PSAM-5HT3 LGICs pair a PSAM domain with a 5-HT 3 serotonin receptor sodium- or potassium-selective ...K + ions, and activates neuronal activity. Figure 3: PSAM4-based ligand-gated ion channels, their effect...routes. Image courtesy of Ute Hochgeschwender . Table 3: Descriptions of LMOs and their activity in neurons...for opsin activation. Proc Natl Acad Sci USA, 113 (3), E358–E367. https://doi.org/10.1073/pnas.1510899113... Huntington's disease pathogenesis. iScience, 28 (3), 112022. https://doi.org/10.1016/j.isci.2025.112022...1157–1167. https://doi.org/10.1038/s41593-020-0661-3 PMID: 32632286 Nakajima, K., & Wess, J. (2012). Design...

...splice donor sequence at its 3' end, while the second vector carries the 3' portion of the gene with a ...protocols, see Addgene’s Viral Vector Protocols . Figure 3: Overview of rAAV vector production. Created with ...frequency which has been estimated to range from 0.01% to 3% depending on cell type and rAAV vector concentration...vectors for human gene therapy . Current Gene Therapy, 3 (4), 281–304. https://doi.org/10.2174/1566523034578285...enhanced gene transfer . Pharmaceutical Research, 25 (3), 489–499. https://doi.org/10.1007/s11095-007-9431...toolkit for human gene therapy . Molecular Therapy, 14 (3), 316–327. https://doi.org/10.1016/j.ymthe.2006.05.009...essential for viral DNA replication and packaging. 3' ITR (RITR) in cis Right Inverted Terminal Repeat,...

...Model for Regeneration and Aging. Gerontology, 66(3), 212–222. https://doi.org/10.1159/000504294 Wang,... more about model organisms in the first Five Popular Model Organisms blog post Read more about plasmids...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...minutes, they are perfect for molecular cloning and metabolic engineering.  The E. coli genome has an extensive...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...characterized genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from...

...thomas-kilmann-conflict-resolution-model/, accessed Jan. 3, 2022.     Additional Resources on the Addgene blog...rely on what I know about those particular teams at that given time. I think about how previous iterations...disagreements. In fact, conflict resolution isn’t just about resolving conflicts between other people, but resolving...normally get along well with each other disagree about a specific decision, direction, or goal. Resolving...of lapsed communication. Making some assumptions about how something should be communicated is necessary...confusion around... Ownership: Misunderstandings about who is responsible for what tasks or projects is...conflicts.  Transitioning a conflict into a collaboration may not be the easiest resolution to accomplish...

...strain. F- Δ(ara-leu)7697 [araD139]B/r Δ(codB-lacI)3 galK16 galE15 λ- e14- mcrA0 relA1 rpsL150(strR) spoT1...thiamine metabolism Requires exogenous thiamine for growth ara disruption of arabinose metabolism pathway...little prokaryotic minions that carried out the labor-intensive process of replicating your new plasmid... Phage resistance gal Mutation in galactose metabolism pathway Cells cannot grow on galactose only ...repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose...plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination...

...recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each varies, it...colonies and check them for successful ligations. Pick 3-10 colonies depending on the number of background ...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...window) New England Biolabs for more information about restriction enzyme buffers). If you select enzymes...

...recognizes a different PAM site and generates 5’ and 3’ staggered ends after dsDNA breaks. The Doudna lab...Addgene Blog Learn about using toehold switches to detect Zika virus   Learn more about targeting RNA with...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...with Cas13a (C2c2) Learn more about Cas12a (Cpf1) and its multiplex genome editing abilities Resources...

...protein." (1998): 509-544. PubMed PMID: 9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters ...deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about 5 from the N-terminus...cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel...based on cofactor binding proteins as described above. The smallest tags are peptides that are engineered...

...Sciences, 24.9 (1999b): 359–363. PubMed PMID: 10470036. 3. Said, H. M. “Biotin: The forgotten vitamin.” American...uses of biotinylation in molecular biology! Learn about in vivo biotinylation of bacterial fusion proteins...covalently attached to the active site of key metabolic enzymes including biotin carboxylase and decarboxylases... organic acids to produce important cellular metabolites. Biotin acts as a carboxyl carrier from one compound...such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the...

...VWR, EX0276-1 Benzonase 250 U/µl, Millipore #71205-3 Polybrene 10 mg/mL, Millipore, TR-1003-G Molecular...mL glutaGRO 50 U/mL benzonase: 15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells...0.03887375114 6.22E+06 2 400 768 18360 0.08366013072 6.69E+06 3 200 1620 15840 0.2045454545 8.18E+06 4 100 3180 20540...the titer. Calculations In the experimental setup above, the following virus dilutions were used to transduce...v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus volume...