We narrowed to 542 results for: SHI

1. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   ...Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National...

2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   ...Hok translation is coupled. Translation from the Shine-Dalgarno (SD) sequence of mok results in translation...

3. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   ...animals are engineered by introducing Cas9-mediated frameshift mutations in the gene of interest. The genomic...

4. The Challenges of Cell Culture

   Type

   Blog Post

   ...cell line provenance and identity. The Nature Publishing Group recently reiterated its strict stance on...

5. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   ...is 20 years old but as you can imagine not old-fashioned at all. With the development of new fluorescent...

6. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   ...used to refer to something that has fallen out of fashion but is still useful and most importantly, effective...

7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   ...PubMed Central PMCID: PMC189872 Schröder, A. R., Shinn, P., Chen, H., Berry, C., Ecker, J. R., & Bushman...

8. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...temperature, allowing for a flow analysis to be shifted to a later day. Note that intracellular labeling...

9. Antibodies 101: Introduction to Gating in Flow Cytometry

   Type

   Blog Post

   ...    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can gate on single...

10. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    ...extended-open. When a ligand binds, it typically shifts the integrin from its closed to its open state,...

11. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    ...double-strand break repair: how to fix a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed...

12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    ...cellular aspects of CRISPR/Cas immune systems in the Sashital Lab, Iowa State University. She is actively interested...

13. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    ...Enterobacteriaceae including in E. coli O157:H7, Shigella flexneri, Enterobacter cloacae, and Serratia marcescens...

14. Five Popular Model Organisms

    Type

    Blog Post

    ... zebrafish reference genome sequence and its relationship to the human genome." Nature496.7446 (2013):...

15. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type

    Blog Post

    ...1630-1640. Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits...

16. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    ...N. L., Puschnik, A. S., Davulcu, O., Diep, J., Ishikawa, Y., Jae, L. T., Wosen, J. E., Nagamine, C. M....

17. CRISPR 101: Off-Target Effects

    Type

    Blog Post

    ...double strand break (DSB), the event required for frameshift and knock-in mutations. If one nickase acts on...

18. Viral Vectors 101: Optogenetic Tools

    Type

    Blog Post

    ...C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H. E., Ganguli, S., & Deisseroth, K...

19. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    ...often leads to the generation of indels and thus frameshifts that disrupt the protein-coding capacity of a...

20. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    ...PMC4663986. 2.  A. M. Singh, V. V Adjan Steffey, T. Yeshi, and D. W. Allison, “Gene Editing in Human Pluripotent...