We narrowed to 771 results for: Dos

1. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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   Blog Post

   ...sharing impact grow beyond COVID-19. But, we can’t do this alone. If you have deposited plasmids at Addgene...

2. Addgene at the ASM Microbe 2016 Conference

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   Blog Post

   ... the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening technology...

3. Seeing Red: Simple GFP Photoconversion

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   Blog Post

   ... and GFP does not display auto-photoconversion as do some FPs. "While this discovery is likely to be most...

4. Future of Research Conference - Remarkable Opening Session

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   Blog Post

   ...for change in their opportunities.  Scientists must do science to be happy so the beginnings of solutions...

5. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   ...could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited to be helping...

6. The Stingy Scientist: How the Baby Gel Box Was Born

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   Blog Post

   ...mindful of the lab materials I was using.  I started to do little things to save reagents and money.  This was...

7. In Living Color: The Skinny on In Vivo Imaging Tools

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   Blog Post

   ...Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

8. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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   ... lentiviral CRISPR library.  “This enables you to do customized genetic modification on a scale that was...

9. Tag Your Favorite Yeast Genes with Ease

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   Blog Post

   ...tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned...

10. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    .... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus...

11. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that...

12. Antibodies 101: Introduction to Antibodies

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    Blog Post

    ...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic...

13. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post

    ...therapeutics. What is antibody engineering (and why do we like it)? Antibody engineering is the modification...ultimately increasing treatment effectiveness. How do you engineer an antibody? There are many, many ways...

14. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first...

15. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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    Blog Post

    ... rhythmic movements like spontaneous swimming. To do so, the lab expressed the LiGluR opsin in different...

16. The Strength of Story Telling

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    Blog Post

    ... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide...

17. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post

    ...why MTAs matter, check out another blog post: Why Do I Need an MTA Anyway?) Don't want to wait? Talk with...

18. Sensing Neuronal Dopamine

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    Blog Post

    ...learning, decision making, and motor control.  What do we understand about Dopamine? DA is produced by specific...

19. Understanding GPCR Signaling Using TRUPATH

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    Blog Post

    ...able to detect extracellular stimuli. One way they do so is through G protein-coupled receptors (GPCRs)...

20. Year of the Camelid: Antibody Style

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    Blog Post

    ...of different cancers (Jin, et al. 2023).  So where do all these sdAbs come from? In the early years of ...