We narrowed to 750 results for: Dos

...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...

...degrading the tissue (Radtke et al., 2020, 2022). I do mean highly multiplexed: you can image over 65 different...

...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...

...sharing impact grow beyond COVID-19. But, we can’t do this alone. If you have deposited plasmids at Addgene...

...arms so that they can hold and hug items closer and do so more animatedly. To communicate that sense of ...

...you high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving...

...much international attention. Their small numbers do not, however, make these patients suffer any less...

...recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety...

... the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening technology...

...for change in their opportunities.  Scientists must do science to be happy so the beginnings of solutions...

...could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited to be helping...

...mindful of the lab materials I was using.  I started to do little things to save reagents and money.  This was...

...Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

...tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned...

...therapeutics. What is antibody engineering (and why do we like it)? Antibody engineering is the modification...ultimately increasing treatment effectiveness. How do you engineer an antibody? There are many, many ways...

...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first...

...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic...

...response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that...

.... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus...

...CRISPR libraries As CRISPR technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also...