We narrowed to 772 results for: Dos

1. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   Blog Post

   ...you high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving...

2. Supporting Rare Disease Research with BeHeard 2017

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   Blog Post

   ...much international attention. Their small numbers do not, however, make these patients suffer any less...

3. SunTag and Fluorescent Imaging

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   Blog Post

   ...recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety...

4. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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   Blog Post

   ...sharing impact grow beyond COVID-19. But, we can’t do this alone. If you have deposited plasmids at Addgene...

5. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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   Blog Post

   ...arms so that they can hold and hug items closer and do so more animatedly. To communicate that sense of ...

6. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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   Blog Post

   ...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...

7. Addgene at the ASM Microbe 2016 Conference

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   Blog Post

   ... the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening technology...

8. Seeing Red: Simple GFP Photoconversion

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   Blog Post

   ... and GFP does not display auto-photoconversion as do some FPs. "While this discovery is likely to be most...

9. Future of Research Conference - Remarkable Opening Session

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   Blog Post

   ...for change in their opportunities.  Scientists must do science to be happy so the beginnings of solutions...

10. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post

    ...could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited to be helping...

11. The Stingy Scientist: How the Baby Gel Box Was Born

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    Blog Post

    ...mindful of the lab materials I was using.  I started to do little things to save reagents and money.  This was...

12. In Living Color: The Skinny on In Vivo Imaging Tools

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    Blog Post

    ...Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

13. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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    Blog Post

    ... lentiviral CRISPR library.  “This enables you to do customized genetic modification on a scale that was...

14. Tag Your Favorite Yeast Genes with Ease

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    Blog Post

    ...tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned...

15. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post

    ...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first...

16. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post

    ...therapeutics. What is antibody engineering (and why do we like it)? Antibody engineering is the modification...ultimately increasing treatment effectiveness. How do you engineer an antibody? There are many, many ways...

17. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    .... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus...

18. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that...

19. Antibodies 101: Introduction to Antibodies

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    Blog Post

    ...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic...

20. Addgene Begins Distribution of Recombinant Antibodies

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    Blog Post

    ...antibodies are excellent for reproducibility because they do not share hybridomas’ genomic drift or limited material...