...AAV Packaged on Request, an expansion of our viral vectors service, has officially launched! As of today... excited for all the ways this much-requested expansion will allow us to better serve our community. If...

... hard about picking a good mentor, and begin considering career options early. Tune in for great advice...year dip (see graph). Niroshi and Ben give their insights as to what causes this dip. 11:11 - 15:37 : How...

...developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single...further to provide multiplexed detection, higher sensitivity, and more. Read the paper in Science Find...

...journals has risen steadily from 1995 to 2013. Considering citations to all articles, Anurag Acharya and...their own libraries and offering another way to consider and quantify the impact of scientific publications...

...tissues, extraction of RNA, precipitation, and resuspension. This protocol provides two options: using Solution...phase without disturbing the interphase layer, consider using a lower volume pipettor like a p200 to collect... freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of it and storing those...

...exemplifies our long-standing commitment to be responsive to the community’s needs and our commitment to...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every...

...ample resources and a positive perspective on the transition to a post-academic career. This training was ... inadequate job of supporting science trainee transition into diverse, successful careers.  We’re not ...

...Directors brings human resources experience and insight to the now 13 member board at Addgene. Let’s meet...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

... It is important to identify your audience and consider their background. Social media is a powerful tool...what you are trying to convey without having to extensively use Google search. Be careful about hidden connotations...

... another level with the addition of two light-sensitive ingredients - CRY2 and CIB1 – which they borrowed...advantages of the new system: "First, the light-responsive molecules of cryptochrome 2 are the chromophores...

...delivery by lentiviral capsid-based bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages...for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the tetraloop of the...

...with cells that already have Cas9). Each library consists of approximately 3,000 gRNAs, as opposed to the...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

...CRISPR-based technique to generate C->T or G->A transitions in a target sequence in live cells. This technique...endonuclease (dCas9) fused to a deaminase to make the transitions at target sequences specified by gRNAs. Caveats...

...traction. One way to consider where the open access (OA) movement might be going is to consider some of the places...

...sequence variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus.... As you develop and confirm new gRNAs, please consider submitting their sequences (and plasmids!) so ...

... the spine. For binders of protocols and data, consider using color coded binders and tabs (for different...to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent storage...

...Foundation are used in Parkinson’s research.  The extensive resource from the Structural Genomics Consortium...Consortium enables science to determine the three dimensional structures of proteins.  The RESOLUTE Consortium...

... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

...solution or a 70% ethanol solution to wipe down the inside of the water bath. Pro-Tip To properly maintain...maximum fill capacity is. Look for a “fill” line inside the water bath or read the user manual. If you’...may float in the water bath when they are placed inside. Water bath floats can be used to secure tubes ...before use, make sure that there is enough water inside....

...for our quality control process because of its expansive feature library and useful tools. In this blog...proteins that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization...