Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 957 results for: REP

Showing: 501 - 520 of 957 results

  1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ... and antibacterial drugs like penicillin and streptomycin. Mycoplasma can pass through filtration methods...the flasks (unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination... but it’s a very good practice for prevention. Prepare a small quantity of media stock that would be used...culture flasks. In cases where the culture is irreplaceable or very expensive, there are some ways to rescue...made permanent damage to the cell culture it is irreparable. Some cells may die due to longer incubation ...

  2. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...Neuroscience Community blog to read reports from bloggers at the meeting – my report is currently in the works....benefit from depositing their constructs to the repository, and getting feedback from our community. It ... on a symposium discussing progress made in reprogramming astrocytes to functional neurons in vivo. And...

  3. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...favorite reagent, scientific technique, or plasmid repository at your next meeting.     If you’re reading...service provides ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some ....         We also have ready-to-use lentiviral preps for COVID-19 research, pooled CRISPR lentiirus libraries...background.       Are none of those backgrounds representative of your favorite technique? Our lab-themed ...

  4. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ...after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble...2008 was marked by the influx of plasmids for reprogramming differentiated cells into pluripotent stem cells...now have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene...

  5. Reflections and Looking Ahead

    Type
    Blog Post
    ...522 organizations in 41 countries enriched our repository with 15,020 new plasmids — a testament to the...viral vector serotypes, or hundreds of Data Hub reports detailing antibody experimental results. We also...options. Almost any AAV transfer plasmid in our repository can now be packaged on demand in five distinct...Antibody characterization is critical to enhance reproducibility in biomedical research. eLife, 13, e100211....

  6. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic Deletions...nucleases to harness non-homologous end joining repair (NHEJ) [1]. Our initial work was with TALENs, in...correlative biochemical characteristics and ectopic reporter assays. We have been interested in exploring the...by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian cells...

  7. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP, with “site” representing the specific...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... strand in the center of the protein. The repeat:anti-repeat duplex pokes out from the REC lobe and doubles....   The REC lobe binds tightly to the repeat:anti-repeat duplex of the guide RNA, confirming its sequence...looking at, let’s peek at unbound—“apo”—Cas9 from Streptococcus pyogenes.   Figure 2:  Crystal structure...

  9. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...tasked with was creating and sustaining a biweekly report of our social media analytics, something that had... meant I was responsible for establishing a new report and routine across the marketing and content team...a fantastic spreadsheet template to build out a report on. I was able to understand what metrics would...larger content and marketing team, and I started preparing for my first official professional presentation...

  10. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...buy a box with the prepaid label and fill it with nitrile gloves, affix the prepaid label, and divert ... lab waste. Despite the fact that researchers represent a minority of the world’s population, we account... (in the U.S.). Their cooler boxes come with a prepaid return shipping label. To return the box, simply...

  11. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ... can prepare you to present your work at conferences, help with manuscript and grant preparation, and ...science communication skills. Ultimately, my degree prepared me to be exactly the type of scientist I wanted...interview with them to learn how their master's degree prepared them for their career.  2. Are you looking for...

  12. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ... can then be purified for subsequent library preparation and high-throughput sequencing. Improvements ...lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the initial CUT... rather than to rely on nuclei extraction. They report that this change offers a significant improvement... in terms of initial steps of cell or tissue preparation.   All in all, CUT&RUN offers a great alternative...

  13. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...detects. This change then generates a reporter signal. Reporter signals may be electrochemical or light-based...light-based, with luminescent and fluorescent reporters being especially popular. We’ll give you an introduction...glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end of the biosensor...

  14. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ... the most frequently requested vectors in our repository and will discuss some of the features you may...more traditional approach of an autonomously replicating vector that carries either a wild-type version...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

  15. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ... perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...protein synthesis Bactericidal 7.5-50 ug/mL Streptomycin aminoglycoside Inhibits initiation of protein...working concentrations. For instructions on how to prepare antibiotic stocks, see Addgene's Reference Page...solution. Storing aliquots at -20oC and avoiding repeated freeze/thaw cycles will keep most antibiotics ...

  16. RUBY-Red Siliques

    Type
    Blog Post
    ...challenging and requires specialized equipment. Reporters for studying gene expression have similar drawbacks...added to the palette. RUBY is a bright red pigment reporter whose expresion is visible to the naked eye in... enzymes and a spontaneous step. (B) RUBY is a reporter consisting of these three enzymes expressed under...References  He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and...

  17. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...frequently denature the proteins as part of the sample prep. This involves using heat and/or chemicals to break...visualize an abstract protein during your sample prep process, imagining how it starts and if any steps...validated for an assay that uses similar sample preparation - for instance, if you cannot find an antibody...for recognizing the protein following your sample prep steps; ie, you may only need to validate on denatured...

  18. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...sequence information, leading to problems with reproducibility and an inability to improve upon these critical...provide renewable reagents with reliable and reproducible production. However, even clonal hybridoma cell...responsibility of the authors and does not necessarily represent the official views of the National Institutes ... References Bradbury A, Plückthun A (2015) Reproducibility: Standardize antibodies used in research. Nature...

  19. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically uses Vaccinia...limit (lower transformation efficiencies have been reported with longer incubation); however, you may need...fairly quick, make sure you stay organized and prepare everything you need for the next step before proceeding... Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions...

  20. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...and Addgenie Angela Abitua hosted a virtual Reproducibility for Everyone workshop at TAGC with the three...engaged through a computer screen. For the Reproducibility for Everyone Workshop, Abitua and the other...knowing you're suppose to draw Spongebob? The Reproducibility for Everyone Workshop included this interactive... activity during their session. Image from Reproducibility for Everyone.  While this type of interaction...
Showing: 501 - 520 of 957 results