...isolate bacteria containing your constructs of interest. But does it matter which antibiotic resistance... retained a functional copy of your plasmid of interest, preventing this issue. The genes and their antibiotics...

...biologists working with vectors are, in most cases, interested in transferring genes to mammalian cells (i.e... the viral RT protein (Faras et al., 1973). Interestingly, retroviruses are generally only “pseudodiploid...

...curve? A poor standard curve leads to over or underestimation of an unknown sample’s concentration. To determine...within the linear portion of the graph. It does restrict the dynamic range of your standard curve, so if...

...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...

...mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences...active in various gram-negative bacterial hosts of interest, including within mixed communities and into species...

...insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly...

...experiments with/for them. Though not the most prestigious role in a company, Technical Support is essential... open to experienced professionals with strong interest in the technical aspects of science and the drive...

...limit the utility of candidate capsids.  Are you interested in using AAV-PHP.B vectors? See Supplementary...B capsids." bioRxiv (2019): 538421. If you’re interested in how PHP.B performed in non-human primates,...

...understandable to scientists outside your field, and interesting. For example, “there is a significant need for...Campus of the Howard Hughes Medical Institute. His interests include the quality control of secretory proteins...

... the ELN is reproducible, and only the highly restricted number of people who can access the ELN have ... the ELN arises here. Notwithstanding access restrictions based on permissions, however, in some cases...

...envelope proteins from another virus to either restrict or broaden the host cell range (tropism). Pseudotyping..., Young JAT, Callaway EM (2007) Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...

...Let's Go! How to Verify Your Plasmid Using a Restriction Digest Analysis Videos What is a Plasmid...Electrophoresis Inoculating Liquid Bacterial Cultures Restriction Digest Analysis We also introduced guides...

... answers to such questions, Addgene is deeply interested in pursuing them. We’ve recently revamped our...can help others as much as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest...

...neurological deficits observed in Rett Syndrome. Restoring the MECP2 gene by excision of the Stop cassette...precise amount of protein to each brain cell to restore physiological levels. Much research is now focused...

...scientists can make mutations throughout a gene of interest based on an organisms codon usage bias to increase...when do you want to codon optimize your gene of interest? Codon optimization has become extremely useful...

...high-throughput next generation sequencing (NGS). Select a restriction enzyme for digest that will cut the shared plasmid...visualize that digestion on an agarose gel ( see restriction digest protocol here ). Lentiviral plasmids can...closed system of a crowded plate or culture. Try restricting growth to the bare minimum by growing at 30 ℃...

...every lab, there are certain hierarchies and restrictions to data access. If these are important in your...Pavlek is a Product Marketing Manager at SciNote, interested in combining scientific research with tech advances...

...could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40% after G418...designing homology arms with the modification of interest, ordering them from a gene synthesis company such...

...protein. SunTag has two components: 1) a protein of interest that’s tagged with 10-24 copies of the short epitope...portion of the receptors recognizes an antigen of interest while the intracellular T cell receptor portion...

...standard curve with a pure sample of your protein of interest (POI). This approach is reliable, but it’s overkill...BioRender.com. On this gel, the protein of interest band is significantly lighter in “condition 1”...