We narrowed to 559 results for: SEC

...that bind the same ligand, making it difficult to dissect the function of one integrin unless others are ...

...Figure 3).  The Hehnly Lab used such a strategy to dissect the roles of three Rab proteins during a key stage...

...here!   Figure 5: Free-floating rat brain sections stained with Anti-GFAP. The staining patterns ...

...to produce scFvs in mammalian, yeast, plant, and insect cells, they are most often expressed from a plasmid...

...their leisure. For virtual B-Roll (that’s the secondary footage you often see playing over the voice-over...

...favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger Krissy...

...participated as a member of the Career Paths Panel on the second day of the symposium and live tweeted throughout...

...annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins in...activity of T5 Exonuclease and furthermore reduces secondary structure of ssDNA. (Rabe & Cepko, 2020). Incubate...

... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

...be more challenging for sequences with complex secondary structure. Today, multiple companies offer long...

...part of the spectrum: PinkyCaMP from the Olivia Masseck Lab (Fink & Imai et al., 2024) and FRCaMPi from...

...Predicting off-target events As mentioned in the above section, there are gRNA softwares available which score...

...may your needed volume (de Backer et al., 2010).  Second, high efficiency transduction coupled with a strong...

...Therefore, in addition to their utility in the health sector, phages represent a reservoir of carbohydrate binding...

...for which we have germline competent ESC lines. Second, the process is much quicker. It takes 3 months...

...and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use...

... in your system. It is very rare for a methods section to include all of the variables that need to be...

...active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells. piggyBac...

...PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control...

...from Pyrococcus furiosus." Acta Crystallographica Section F: Structural Biology and Crystallization Communications...