We narrowed to 560 results for: SEC

...that bind the same ligand, making it difficult to dissect the function of one integrin unless others are ...

...Figure 3).  The Hehnly Lab used such a strategy to dissect the roles of three Rab proteins during a key stage...

...dialing it in took a lot of trial and error. The second hard part was making CASPER impactful to the community...

...The PAM sequence near this site is AGC, so in a secondary screen, the team tested Cas9 mutations that imposed...

...dBrainbow includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce...

...here!   Figure 5: Free-floating rat brain sections stained with Anti-GFAP. The staining patterns ...

...participated as a member of the Career Paths Panel on the second day of the symposium and live tweeted throughout...

... Oftentimes, the water bath will have a spot to secure the thermometer in place. Turn the water bath on...placed inside. Water bath floats can be used to secure tubes in place and water bath weights can hold ...

...annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins in...activity of T5 Exonuclease and furthermore reduces secondary structure of ssDNA. (Rabe & Cepko, 2020). Incubate...

...Therefore, in addition to their utility in the health sector, phages represent a reservoir of carbohydrate binding...

...for which we have germline competent ESC lines. Second, the process is much quicker. It takes 3 months...

...and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use...

... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

...be more challenging for sequences with complex secondary structure. Today, multiple companies offer long...

...Predicting off-target events As mentioned in the above section, there are gRNA softwares available which score...

...may your needed volume (de Backer et al., 2010).  Second, high efficiency transduction coupled with a strong...

...part of the spectrum: PinkyCaMP from the Olivia Masseck Lab (Fink & Imai et al., 2024) and FRCaMPi from...

... in your system. It is very rare for a methods section to include all of the variables that need to be...

...active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells. piggyBac...

... was interested in microbes until the end of my second year! Conclusion and other pieces of advice Give...