We narrowed to 556 results for: SEC

...The PAM sequence near this site is AGC, so in a secondary screen, the team tested Cas9 mutations that imposed...

...here!   Figure 5: Free-floating rat brain sections stained with Anti-GFAP. The staining patterns ...

...favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger Krissy...

...participated as a member of the Career Paths Panel on the second day of the symposium and live tweeted throughout...

...annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins in...activity of T5 Exonuclease and furthermore reduces secondary structure of ssDNA. (Rabe & Cepko, 2020). Incubate...

...be more challenging for sequences with complex secondary structure. Today, multiple companies offer long...

...Predicting off-target events As mentioned in the above section, there are gRNA softwares available which score...

...may your needed volume (de Backer et al., 2010).  Second, high efficiency transduction coupled with a strong...

...part of the spectrum: PinkyCaMP from the Olivia Masseck Lab (Fink & Imai et al., 2024) and FRCaMPi from...

... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

...Therefore, in addition to their utility in the health sector, phages represent a reservoir of carbohydrate binding...

...for which we have germline competent ESC lines. Second, the process is much quicker. It takes 3 months...

...and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use...

...also happen at the state and local level. The big secret about science policy that I didn’t learn until ...

...from Pyrococcus furiosus." Acta Crystallographica Section F: Structural Biology and Crystallization Communications...

...fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins to make better...

... in your system. It is very rare for a methods section to include all of the variables that need to be...

...active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells. piggyBac...

... was interested in microbes until the end of my second year! Conclusion and other pieces of advice Give...

...through Addgene’s viral service and the methods sections of scientific articles (gasp). To my delight, ...