We narrowed to 609 results for: SHE

1. Antibodies 101: Conjugation

   Type

   Blog Post

   ...conjugated antibodies that are ready to use off the shelf. So why are we talking about this? Even though there...

2. To Each HIS Own

   Type

   Blog Post

   ...used for a variety of assays in the lab (for a refresher see Antibodies 101: Introduction to Antibodies...

3. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   Blog Post

   ...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types...

4. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   Blog Post

   ... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt...

5. Plasmids 101: Cre-lox

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   Blog Post

   ... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse...

6. General Transfection

   Type

   Protocol

   ...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

7. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...compatibly digested vector backbone. This is accomplished by covalently connecting the sugar backbone ...

8. Engaging with science and society at pgEd

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   Blog Post

   ...as a society are to figure out the best way to shepherd technologies through thorny ethical issues, ensure...

9. Lab Automation at Addgene

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   Blog Post

   ...would be much easier with a Tube Writer, which air brushes the information onto the vials instead of requiring...

10. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    Blog Post

    ...Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 ...

11. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post

    ... Fogarty, E. C., Schechter, M. S., Lolans, K., Sheahan, M. L., Veseli, I., Moore, R. M., Kiefl, E., Moody...

12. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

    ...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...

13. How to Prepare for an Industry Interview

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    Blog Post

    ...lunch on your schedule, but it will probably be rushed and you’ll have to answer questions between bites...

14. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post

    ...work has focused on modifying the outside protein shell, or capsid, of the virus so that it only enters ...

15. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    Blog Post

    ...strategy that uses genetically engineered mice. Best wishes on your experiment! References Becher, Burkhard...

16. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

    ...wells while the protein-antigen complexes will be washed away during the protocol. The amount of antibody...

17. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in...

18. Plasmids 101: Modular Cloning

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    Blog Post

    ... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

19. Viral Vectors 101: Calcium Sensors

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    Blog Post

    ...on! References and Resources References Nasu Y, Shen Y, Kramer L, Campbell RE. Structure-and mechanism-guided...

20. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical...