...that infectious wild-type viruses were produced in high-titer retroviral stocks generated by the transfer...In this kind of approach, a population of cells might express only the tTA or your transgene resulting...developed, but these vectors are quite large and you might get lower viral titer when using them.   Regarding.... If you are transducing non-adherent cells you might need to centrifuge your cells with the virus in ...Trouble Shooting Viral Transductions Learn Why You Might Want to Use AAV in Your Research Lentiviral Vector...

...unless the source of culture is irreplaceable or highly expensive). Detecting mycoplasma contamination ...induces a noticeable color change. This method has high sensitivity but low specificity (Degeling, 2012)...required in this process because result interpretation might be difficult. The low density of mycoplasmas, contamination...media as much as possible and the ones that are tightly packed with seals. Use disposable pipettes to avoid...exposure of the cell culture to air and make sure to tighten the flask cap before placing them in the incubators...

...decades of my life following a very straightforward conveyor belt from high school to college to graduate school...Veterans Health Administration, I could never get one straight answer about what science policy even is! In this... “little p” policy.  “Big P” policy is what you might typically think of. It occurs in a government setting... challenging if you are trying to apply for an outright job in a field where you have no specific scientific...

...and four chaperones from Denis-Yarmouth Regional High School visited our labs in Cambridge, MA to learn... Outreach Scientist Jessica Welch showed some highlights of his travels from 2015, including a great European...Website Learn more about Dennis-Yarmouth Regional High School Resources at Addgene  Read more Inside ...

...working with AAV or lentivirus in your research, you might want to use one of our virus backgrounds. Addgene...backgrounds for you as well! If you use FRET, you might be interested in our fluorescent protein background....           Did you get the joke? If not, you might want to check out Addgene’s Fluorescent Proteins...background.     We hope these science backgrounds will brighten up your virtual meetings!  ...

...It’s well known that exercise can help fight stress and depression, and now researchers have reported...including unpredictable loud noises and flashing lights - that sent average, control mice into depression...know about you, but I think I could use a good run right about now. And, if you are looking to get your hands...for studies in your own lab, you’ve come to the right place. Get started with PGC-1 plasmids from Addgene...

...Directors brings human resources experience and insight to the now 13 member board at Addgene. Let’s meet...hope Addgene will accomplish in the near future? Right now it’s a matter of maneuvering through the impact...volunteer appointments included the Belmont Human Rights Commission and mediator for the Massachusetts small... dedicated to volunteerism and ran the Mayor’s Midnight Sun Marathon in Anchorage, for the Leukemia Society...

...to the field. Fortunately, our bodies have been fighting cancer for far longer than we’ve been studying...natural abilities, we’re gaining new ground in the fight against cancer in its many forms. The dichotomous...cancer stops growing, and the immune system stops fighting. Such quiescent cancer may rear its head once ...infection can further divert the immune system from fighting cancer. Surgical removal causes increases in tumor... a sequence of immune reactions that aid in the fight against cancer. Sipuleucel-T by the Dendreon Corporation...

...instance, you might use it as part of a flex switch you’d like to work with in neurons or you might use it in...Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre Of course...brain. There are many possibilities enabled by this highly modular AAV - that’s probably why it’s so popular...

...bigly. But such a project should not be undertaken lightly. Whether executed in arrayed or pooled format there...are just as critical for success. Sometimes, you might have a few positive control genes on which to test...execute genome-wide perturbations. So, while some might argue that the only way to do something is to do...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

...group? The Ellis Lab self-organizes cinema nights, game nights, and trips to eat out together. “We also ... celebrate your lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams successes...

...? If so, you might want to find additional sources of wisdom. While somewhat lighthearted, the article...article does highlight key characteristics of a good mentor: Yoda image courtesy of jorenl/Vecteezy.com...

...share? And how they are connected? Here are the highlights written as 8 tweetable facts. Note: The images...most Twitter users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines on Twitter. ...disciplines: social (left), life (center), and physical (right). How do you use Twitter as a scientist? If you ...

...them share their reagents, we knew our role in fighting the pandemic could be even more impactful by reaching...scientists working in a for-profit setting.  In light of the need for basic research during the current...University of Iowa University of Minnesota See highlights from the COVID-19 collection Partnership with...

...Agrobacterium, along with a selectable marker. Left and right T-DNA borders specify the region of DNA that will...codon-optimizing the Agrobacterium ORI, they achieved much higher DNA yield and transformation efficiency in both...that gene expression from the M24 promoter is much higher than that from the commonly used 35S promoter. ...transfer. The M24 promoter provides constitutive, high-level gene expression and is functional in a variety...

...been in space is pretty small). Therefore, I was delighted to be invited to the ceremony for surely what ...non-scientist from Bloomberg news. Given the topic it might have been better to have a scientist up there, but... viruses to give information to the computer to fight the known viruses. Bacteria have figured out how... virus sequence to enable them to recognize and fight it forever. He emphasized the potential of this ...

...research". The family entered the biomedical field’s spotlight after researchers discovered heavy-chain only ...reading frame Ability to access unique epitopes with high affinity, due to a greater ability to target concave...cellular functions, like in this early example we highlighted back in 2015, where sdAbs were used to create...

...toxic to both lines, it probably has either high toxicity or high off target effects, both of which make it... druggable target). Diseases with defined, straightforward causes (a specific mutation in a known gene...targets by CRISPR can accelerate the drug pipeline. High-throughput CRISPR screens can be used to inhibit...

...Gates’ Foundation is involved with was one of the highlights of the conference. It was inspiring to see how...trusty spray bottles! We also raffled off one of our highly sought-after "nice genes…" tshirts, and the lucky...Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday morning, John Doench’s opening...

...skills to get the work done efficiently and with high quality. Their work involves coordination and can...t mean going rogue, pointing out problems or highlighting criticisms. To be given the opportunity to take... by Peter Fuda and Richard Badham Leadership Insights for Engineers, Scientists and Computer Professionals...