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We narrowed to 724 results for: rel

Showing: 501 - 520 of 724 results
  1. Bioinformatics at Addgene

    Type
    Blog Post
    ... creating a robust data pipeline that would be reliable and available for our teams to use, one of our...container for its particular purpose and knew we could rely on the containers to produce the same output regardless...
  2. The Challenges of Cell Culture

    Type
    Blog Post
    ... so any results generated are reproducible and reliable. When you order a new cell line you will need ...conducted during projects to ensure the results are reliable. With this two-pronged approach, the many mistakes...
  3. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ... protein of interest’s function. This technique relies on the generation of a plasmid library that expresses...mutagenesis (SDM) PCR, but with a twist since it relies on the sequential nicking, degradation, and PCR-generation...
  4. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...integration for their life cycle to continue. They rely on a viral protein called reverse transcriptase ...lentiviral vectors. This is because most titering methods rely on isolating genomic DNA from transduced cells and...
  5. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...a fluorophore that is quenched when intact and released when cleaved. Cleavage results in fluorescence...on collateral cleavage by the enzyme and instead relies on its activity on the target regions thus making...
  6. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...question? This should be something that anyone could relate to, that people would agree is important to understand...
  7. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... lab, Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version...measurement of different RTKs. Although this sensor relies on a change in localization to the cell membrane...
  8. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... was carefully chosen to perform statistically relevant benchmarking of cell-free expression systems, ... the efficiency of their cell-free system in correlation to the size of the product. Analysis of protein...
  9. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...etc. This will help save time by screening out irrelevant candidates, and, more importantly, it’ll help... try to keep conversations focused on research relevant objectives and their tangible resources. For example...
  10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...general, epigenetic chromatin modifications are correlated with alterations in gene expression, but causality...tools for this purpose. Epigenetics began as a correlative field in which covalent modifications to DNA ...
  11. A Control for All Seasons

    Type
    Blog Post
    ...controls and then moving on to controls for antibody-related applications. After reading, you will have a better...and test multiple mice with the drug rather than rely on the results of a single mouse. Biological replicates...
  12. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...to growth factors, some of the most clinically-relevant proteins are also the hardest to study. One common... improve more physiologically and endogenously relevant models, tools that increase confidence become ...
  13. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... procedure is cumbersome and inefficient, as it relies on random integration efficiencies that may be ...off-target effects is likely due to the fact that PBx relies on the inverted terminal repeats (ITRs) found on...
Showing: 501 - 520 of 724 results