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TypeBlog Post...would be much easier with a Tube Writer, which air brushes the information onto the vials instead of requiring...
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Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...strategy that uses genetically engineered mice. Best wishes on your experiment! References Becher, Burkhard... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post... Fogarty, E. C., Schechter, M. S., Lolans, K., Sheahan, M. L., Veseli, I., Moore, R. M., Kiefl, E., Moody... -
Plasmids 101: Modular Cloning
TypeBlog Post... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology... -
How to Prepare for an Industry Interview
TypeBlog Post...lunch on your schedule, but it will probably be rushed and you’ll have to answer questions between bites... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...wells while the protein-antigen complexes will be washed away during the protocol. The amount of antibody... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...on! References and Resources References Nasu Y, Shen Y, Kramer L, Campbell RE. Structure-and mechanism-guided... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...work has focused on modifying the outside protein shell, or capsid, of the virus so that it only enters ... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post... a project forward, transductions should not be rushed into. Before you start any transduction, you should... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates... -
Lentiviral Vector Guide
TypeGuide... you're new to viral vectors or simply need a refresher, we've included a glossary at the end! Browse ... -
3D Printing Meets CRISPR Cas9
TypeBlog Post...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions.... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post... is generally advised, there is little detail published on how to do it best. Scientists are typically... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post... Alyssa Shepard...