We narrowed to 540 results for: try

1. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   ... single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID: 23495938. Additional...

2. Tips for arabidopsis transformation

   Type

   Blog Post

   ...Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi: 10.1271/bbb.70216...

3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

   Type

   Blog Post

   ...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...

4. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   ...lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage...

5. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   ...such reagents as outlined by your institution or country. Have any other questions? Feel free to post them...

6. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   ...Next-Generation Sequencing Technologies. Analytical Chemistry, 83(12), 4327–4341. https://doi.org/10.1021/ac2010857...

7. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   ...Initiation of Replication. Journal of Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc...

8. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   ...-based biosensors.” TrAC Trends in Analytical Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina...

9. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   ... Basics of Western Immunoblotting.) For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively...

10. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    ...virus is to delete the glycoprotein required for entry into the cell (rabies dG). Because rabies dG can...

11. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    ...fluorescence that can be visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine...

12. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    ...going to graduate school, Joe taught high school chemistry for a year. The teaching/mentoring bug never went...

13. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    ... into the desired repository. They also support entry of required metadata, with the option of adding ...

14. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...

15. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    ...the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

16. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    ... barrier (BBB). It’s the BBB’s job to block the entry of pathogens to the CNS, but this also stops viral...

17. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS.  Thus, the ...

18. Important Considerations in Optogenetics Behavioral Experiments

    Type

    Blog Post

    ...neurons: differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID...

19. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    ...gene-silencing machine. Journal of Biological Chemistry, 284(27), 17897–17901. https://doi.org/10.1074...

20. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    ...characterization of dedicated sensors for FLIM, for ratiometry and with high affinity." PloS one 10.4 (2015)...