We narrowed to 540 results for: try

1. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   Blog Post

   ...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed...

3. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   Blog Post

   ...s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and...

4. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   ...lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage...

5. Your Lentiviral Plasmid FAQs Answered

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   Blog Post

   ...such reagents as outlined by your institution or country. Have any other questions? Feel free to post them...

6. Neuronal labeling with Spaghetti Monster

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   ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...

7. Fluorescent Proteins 101: Aptamer Fluorophores

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   ...-based biosensors.” TrAC Trends in Analytical Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina...

8. Deciphering the Mysteries of Behavior with Viral Vectors

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   ...the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

9. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ... barrier (BBB). It’s the BBB’s job to block the entry of pathogens to the CNS, but this also stops viral...

10. New Tools Enable CRISPRa for Neuroscience Applications

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    ... visualizing neuronal differentiation by immunohistochemistry, it is not amenable to FACS.  Thus, the ...

11. Plasmids 101: Broad Host Range Plasmids

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    ...Initiation of Replication. Journal of Biological Chemistry, 273(14), 8447–8453. https://doi.org/10.1074/jbc...

12. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    ...fluorescence that can be visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine...

13. Antibodies 101: Immunoprecipitation

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    ... Basics of Western Immunoblotting.) For mass spectrometry, elute in a urea-containing lysis buffer. Alternatively...

14. Viral Vectors 101: Pseudotyping

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    ...virus is to delete the glycoprotein required for entry into the cell (rabies dG). Because rabies dG can...

15. QC Sequencing Technologies at Addgene

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    ...Next-Generation Sequencing Technologies. Analytical Chemistry, 83(12), 4327–4341. https://doi.org/10.1021/ac2010857...

16. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...going to graduate school, Joe taught high school chemistry for a year. The teaching/mentoring bug never went...

17. Supporting Reproducibility with a Connected ELN

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    ... into the desired repository. They also support entry of required metadata, with the option of adding ...

18. Important Considerations in Optogenetics Behavioral Experiments

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    ...neurons: differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID...

19. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

    ...Single Chain Fv Protein." Journal of Biological Chemistry (2000). PubMed PMID: 10748019.  8. Lotze, Jonathan...

20. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    Blog Post

    ...effective, in both academic circles as well as in industry. The news is good. In their ideal form, ELNs provide...