We narrowed to 569 results for: CIT

1. Addgene's Cancer Collection Pages Connect the Research Community

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   ...residues. For Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway...

2. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an A206K mutation [2]....

3. Genome-wide Screening Using CRISPR

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   ...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...

4. Viral Vectors 101: The Retroviral Lifecycle

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   ...mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia...

5. Uncovering Ancestral Bias in CRISPR Research

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   ...their associated ancestries. While there were some exciting relationships identified, 48% were found to be...

6. DNA Purification Without a Kit

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   ...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...

7. Transferable Skills Guide: Identifying Your Transferable Skills

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   ...satisfaction out your work, and be prepared for new and exciting opportunities. Here are some strategies for identifying...

8. Simplify Cloning with in vivo Assembly

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   ...molecular biology of synaptic transmission and plasticity.     Javier García-Nafría is an investigator ...

9. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...

10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...

11. Tips for Screening with Yeast Two Hybrid Systems

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    ...eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell. 1985; 43:729...

12. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...your event in casual conversation to get people excited!    Laura Veckerelli is the Administrative and...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...proteins and CRISPR became available, I was very excited because genome-editing with these molecules makes...

14. CRISPR 101: Any Base Transversion Editors

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    ...tool vary considerably. And there’s so much more exciting protein engineering going on in the base editing...

15. Viral Vectors 101: Preparing Pooled Libraries

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    ...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...

16. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    ...concentrations (0.1-1% L-arabinose) without signs of toxicity or burden relative to a fluorescent protein control...

17. Antibodies 101: Immunoprecipitation

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    ... of interest. The wide availability and high specificity of anti-epitope antibodies make this approach...

18. CRISPR-based Genome Editing Systems For Editing in E. coli

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    ... B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized ...

19. Transferable Skills Guide: External Collaborations

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    ...Partnership Management, a very interesting and exciting part of my work. However, this is not the first...

20. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...molecules, which could bind their targets with high specificity and affinity, were eventually called aptamers...