We narrowed to 575 results for: CIT

...mismatch tolerance and, consequently, increased specificity relative to the more commonly employed 20nt gRNAs...

...Flp-dependent DREADDs viral vectors. Both inhibitory and excitatory DREADDS from the Gether lab are now available...

... locus. The two methods had similar rates of specificity for the DNA sequence targeted in the experiment...

... a few different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal...

...using different Type IIS restriction enzyme specificities at each step. Factors such as enzyme efficiency...

...residues. For Ras researchers, one of the most exciting and relatively new resources is the RAS Pathway...

...CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine) can be monomerized with an A206K mutation [2]....

...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...

...mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia...

...stacked together in series to increase binding capacity. Figure 1: Purify DNA using glass filters...

...satisfaction out your work, and be prepared for new and exciting opportunities. Here are some strategies for identifying...

...molecular biology of synaptic transmission and plasticity.     Javier García-Nafría is an investigator ...

...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...

...their associated ancestries. While there were some exciting relationships identified, 48% were found to be...

...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...

...eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor. Cell. 1985; 43:729...

...your event in casual conversation to get people excited!    Laura Veckerelli is the Administrative and...

...proteins and CRISPR became available, I was very excited because genome-editing with these molecules makes...

...tool vary considerably. And there’s so much more exciting protein engineering going on in the base editing...

...no more than one viral particle, giving a low multiplicity of infection (MOI). MOI is controlled by viral...