...containing gaps or DNA base pair mismatches (8-11). For some applications, there cannot be any end-joining activity...those containing one or more mismatches. However, some ligases can ligate mismatches to a significant degree...pyrimidine:purine mismatches. Helix stability also plays some role, and mismatches with more hydrogen bonds are...

... themselves from one location and then insert somewhere else (Fig. 2). Class II TEs have LTRs on both ...Applications of transposons Now that you’ve learned about some of the popular transposon systems, let’s take a ...and cell therapy, and have potential to overcome some of the limitations of viruses. TEs have a large ...

...therapies themselves can be made more effective. Some important factors researchers are beginning to focus...broadly activate the immune response against tumors. Some chemotherapeutic agents induce immunogenic tumor...deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death receptor...

...swarming around S. aureus and eating the bacteria. Some of the many questions scientists have been able ...used in your experiments, experience has shown that some are more reliable and more efficient at tracking... Tips on Choosing Your Fluorescent Protein Learn Some of the Pitfalls of Working With Fluorescent Proteins...

...approaches are commonly used to produce KO cell lines, some of which are commercially available. Various proteomic...multiple techniques. For example, we have found that some antibodies perform well in a PFA-saponin fixation...). The approach maps onto the five pillars, with some specific recommendations for how to use these in...

...will deliver some fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping...

...on the basics of viral vectors, applications, and some new resources available from Addgene. There’s a ...vectors In addition to “going viral,” we also have some other exciting news. Last week, we launched our ...

...after all a place that encourages people to take some time out every once in a while – just read Melina... people in a better mood. Laughter itself can sometimes even count as demanding exercise, and, of course...

...available online if you prefer the DIY method. As some proteins aggregate at 95 °C, you may want to check...preserve sample, check the ingredients before using, as some contain Tween-20, which can strip proteins from ...non-specific binding; lot-to-lot variability Some recombinant antibodies Not always available; not...

...received a festive makeover today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them... to everyone who decked their lab and brought us some holiday cheer!   ...

...export license application for rabies materials! Some of the materials in our repository, including rabies...license for rabies materials may be required for some countries and may not be required for others. You...

... about their career paths, future goals, and get some useful career advice.  Our first video, featuring...and interesting content. Wish we had a video on something that you don’t see here? Give us suggestions in...

...3 choices), we'll be in touch with you soon with some brand-new Addgene swag!   Last month, we introduced...submission is included in the final four will receive some rockin’ newly designed Addgene swag. Good luck and...

...serotype AAV-hSyn-Soma-jGCaMP8s AAV9 Biosensor Fyhn New serotype AAV-hSyn-Soma-jGCaMP8f AAV9 Biosensor...

... I went to college for microbiology and then did some independent research my junior year and discovered...other companies. It's one of those "every day is something different" type of jobs, which really suits my...

..., welcome to our new and improved site! Here are some of the exciting new features on the blog that will...will give you a better experience. Looking for something? Use the navigation menu. The new navigation menu...

...graduate student life. Senior graduate students can sometimes be elusive or have a certain mystique that makes...University of California, Los Angeles. She enjoys chromosome biology and reading Ben Vincent's tweets.    ...

...yet read the following Top 10 posts of 2014, take some time and see what you’re missing. Let us know if...holiday season and we look forward to seeing what awesome research you and your labs produce in the new year...

...likely talked to one of us! Today we’re discussing some of our most frequently asked questions related to...payment during checkout. Placing your order online has some benefits. All of the information that is entered...

...sequencing reactions a week. So I have developed some good habits that I wanted to pass on to you to make...reaction (as opposed to using the all inclusive service some companies offer), you can purify the product either...