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... In 2012, Casey Maguire and colleagues from Massachusetts General Hospital and Harvard Medical School ...behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs have improved transduction profiles...in target cells and areas that are difficult to reach, and are more resistant to inactivation through ...

...was very popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held...as a nonprofit organization in the state of Massachusetts. Because we’re conveniently located next to ...wants to share his knowledge with the community by teaching a DIY plant engineering workshop. Community projects...

...NNGRR(N)  Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium (LbCpf1) 5' TTTV AsCpf1 RR variant...below). After years of research, we now know that each direct repeat, combined with its adjacent spacer...unique, foreign DNA target sequences specific to each individual crRNA. Bottom line, the “direct repeat...

...’s manual for the optimal working conditions for each enzyme. Watch out for methylation issues. Enzymes.... Bonus: knowing how much DNA you have loaded in each well will allow you to approximate the DNA mass ...however, you may need to run the gel for longer to achieve good separation of larger DNA fragments. I hope...

... an off hands environment? Does the group expect each other to be physically present at certain times ...sure your advisors will be good role models and teach you to be the scientist you want to be. For much...Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia  Advice for Scientists Starting...

... are here to discuss the ins and outs of both approaches. Screens and selections The common goals The...details - and that’s where the differences in these approaches can be found. As an example, say you are considering...and consider these questions to save yourself a headache later on. Enhancer and suppressor screens These...

...020-0600-6. The Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder... which also provides a useful yet low-pressure teaching opportunity for lab members or students new to...for mammalian genome regulation and editing by: Rachel Leeson The large size of CRISPR-Cas effectors and...

...points. Many thanks to our guest blogger Rachel Rubinstein! Rachel Rubinstein is currently a Field-based ...This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager...

... personnel from the industry and brainstorm with each other. The event also featured a seminar on “Tools...scientific reproducibility” by Benoit Giquel, an outreach scientist from Addgene. The talk highlighted the...research.  We’ve also involved ourselves in extensive outreach programs to encourage and embolden the spirit ...

...types or signals in the brain. For this to work, each indicator needs to emit a different color of light...Less signal distortion from photoswitching or photobleaching Improved signal-to-noise ratio (SNR). ...Tool: The iGluSnFR3 Glutamate Sensor A Practical Approach to Choosing the B(right)est Fluorescent Protein...

...CRISPR array yielded >90% knockdown by CasRx for each of four targets, including two mRNAs and two nuclear...which efficiency varies widely across guide RNAs, each guide tested with CasRx yielded >80% knockdown. ... exon skipping. Previous CRISPR exon-skipping approaches used two guide RNAs to remove a given exon from...

...a lot of jargon and have a technical, detailed approach. Other times, it is more helpful to use plain ...text for their practice image. We know; we asked each group to share with the rest of the workshop.  For...muddled due to the passage of supernatant through each layer. The right panel illustrates a gradient tube...

...First, it needs to be virally enveloped which is achieved by expression of the vesicular somatitis virus...within the viral particle. The ITRs base pair with each other to allow DNA synthesis of the complementary...and gamma-retroviruses have a lot in common with each other. Below we will outline the common features...

...methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) Most rely on transfection... One vector expresses Cas9 and up to 7 gRNAs Each gRNA requires its own promoter Sakuma et al   ...One vector expresses Cas9, eGFP, and up to 4 gRNAs Each gRNA requires its own promoter   Kabadi et al ...

...into two fragments (BotNT-N and BotNT-C) and joined each fragment with one part of an established pair of...collection consists of a library of reporter plasmids, each containing two fluorescent proteins and four repeats...Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used...

...with Addgene? Katz: Joanne and I first got to know each other through the Healthcare Businesswomen’s Association...Paratore: Kristen Darby, who’s also a board member, reached out and asked if I would be interested in joining...responsible for bringing a systematic and disciplined approach to evaluating and improving the effectiveness ...

..., repressing RecA and overexpressing the NHEJ machinery improved NHEJ accuracy in M. smegmatis (Yan et...also be engaged by variants of the canonical Cas9 approach. A pair of CRISPR guides that flank regions of... break; when two such breaks are introduced near each other, in opposite strands. This “double nickase...

...widely utilized across many different cell types and each one requires different elements for vector propagation...control not only the number of plasmids found in each cell, but also whether the plasmid gets integrated...non-physiological levels. Scientists have tried varied approaches to combat these issues. One method to reduce ...

...you can easily distinguish each different type of antibody and therefore each target protein. This can ...fluorophore-conjugated secondary antibodies specific to each isotype or species to easily discern the proteins...

...identical heavy chains and two identical light chains) each with a constant region and a variable region. The...regions, both through genetic and proteolytic approaches. This comes in handy when, for example, you want...product is a mini-Y with both Fab regions still attached to one another. Size: ~110 kDa. Fab' - These fragments...