We narrowed to 569 results for: CIT

1. CRISPR 101: Any Base Transversion Editors

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   ...tool vary considerably. And there’s so much more exciting protein engineering going on in the base editing...

2. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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   ...concentrations (0.1-1% L-arabinose) without signs of toxicity or burden relative to a fluorescent protein control...

3. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ... B strains, 1 E. coli W strain, and 1 Tatumella citrea, with a total of 70 sites edited. We optimized ...

4. 5 Reasons to Use Reddit for Science Communication

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   ...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...

5. Getting the Most from Your Lentiviral Transduction

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   ...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

6. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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   ...fusion with your favorite protein of interest is exciting and rewarding. But, what if an FP fusion with ...

7. Transferable Skills Guide: Time Management

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   ... progress. For this, I always make sure I have explicit goals in mind for the endpoints of a task or project...

8. Five Popular Model Organisms

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   ... similar to humans. For example, due to their simplicity, yeast have become an important experimental ...

9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ... fluorescence can be observed under either no excitation light, UV light or LED blue light.  Highlights...

10. Transferable Skills Guide: Conflict Resolution

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    ...resolvable, but will likely require a manager to explicitly assign the responsibility in question to a specific...

11. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    ...therapeutic and imaging applications; and reduced immunogenicity when administered in vivo due to their lack...

12. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ... is distributed across multiple plasmids. For simplicity, we’ll use the second-generation system as an...

13. Hot Biosensors 2022: Year-End Roundup

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    ...significantly outperforms previous designs. B-gTEMP is excitable under blue light (470-495 nm) and is capable of...

14. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ...biotin labeling in 10 minutes without any issues in toxicity. TurboID’s signal is significantly greater than...

15. Multiple Plasmids at a Low Price: Kits from Addgene

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    ... RNA endonucleases with customized sequence specificities." Nature communications 3 (2012): 1147. Cooke...

16. Antibodies 101: Multiplex Immunofluorescence

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    ...combination of host and target species, isotype specificity, and dye meets your needs and budget, or you ...

17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ... et al. "Permanent genetic memory with> 1-byte capacity." Nature methods 11.12 (2014): 1261-1266. PubMed...

18. Generating Mouse Models Using CRISPR/Cas9

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    ...a cut at the target site. This is when it gets exciting – the cell sends out an “SOS” signal and cellular...

19. Quick Guide to All Things Lentivirus

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    ... incubate your cells with virus at different multiplicity of infection (MOI=ratio of virus to cells). ...

20. Finding Your Science Policy Path

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    ...graduate school via this report from the Scientific Citizenship Initiative.    Get practical experience in science...