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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 557 results for: SPR

Showing: 541 - 557 of 557 results

  2. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTCTGGGCTCGATCTTGAgctcgatggaaaaaccTCCAACCGTTCACGATCA spry4 TAL3182 & TAL3183 TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...producing their own INTRSECT plasmids. Online spreadsheet (Link opens in a new window) of Flp-recombinase-expressing...

  6. Plan Your Experiment

    Type
    Guide
    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan Your Experiment...you started using CRISPR in your research. Although we will use the example of CRISPR/Cas9 in mammalian...express the CRISPR machinery. For more difficult cells (e.g. primary cells), viral delivery of CRISPR reagents...conducting genome-wide screens using CRISPR AAV transduction CRISPR elements are inserted into an AAV transfer...lipids Transient expression of CRISPR components Short window of CRISPR activity may decrease off-target...Expression System Design gRNA Publications Get Started CRISPR is a powerful system that enables researchers to... cells, many of these principles apply to using CRISPR in other organisms. First, consider the genetic...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our...of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE... plasmids have been depleted by selection. In a CRISPR screen, negative screens are often used to identify...

  8. Addgene's eBook Collection

    Type
    Guide
    ...Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | ...Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español...experimental advice such as tips for planning your CRISPR experiment and designing your gRNA and how to use...traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 ...

  9. Science Guides

    Type
    Guide
    ...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient...Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...and base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science...

  11. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...size, low immunogenicity, and limited ability to spread after injection. Different AAV serotypes exhibit...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase...

  12. Molecular Biology Reference

    Type
    Guide
    ...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and ...the genome. For more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for...

  13. Educational Resources

    Type
    Guide
    ...to our collections. Titles include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 ...include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology Career Readiness...in-depth instructional content on subjects such as: CRISPR Chemogenetics Optogenetics Genetic Code Expansion...

  14. Chemogenetics Guide

    Type
    Guide
    ...different AAV serotypes have varying degrees of viral spread within injected tissue, and this must be considered...especially useful in AAV experiments where the virus can spread around the injection site and potentially express...

  15. Adenovirus Guide

    Type
    Guide
    ...of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors...Production and Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...

  16. Optogenetics Guide

    Type
    Guide
    ...tools for optical control of neural activity. Cold Spring Harb Protoc. 3:top102. PMID 21363959 Yizhar O, ...

  17. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Retroviral Vector Design. In Retroviruses . Cold Spring Harbor Laboratory Press. NIH Bookshelf De Ravin...
Showing: 541 - 557 of 557 results