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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 562 results for: Spr;

Showing: 541 - 560 of 562 results

  1. Synthetic Biology - Overview

    Type
    Collection
    ...Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins FREQ-Seq Kit FX Cloning Kit...

  2. AAV Molecular Tools

    Type
    Collection
    ...Tools These AAV encode Cas9 nuclease used in the CRISPR-Cas9 system for targeted genome editing. ID Name...

  3. Promega Plasmid Collection

    Type
    Collection
    ...regulation, tracking protein degradation, analyzing CRISPR-mediated gene editing outcomes, and conducting ...

  5. p53 Pathway

    Type
    Collection
    ...gain-of-function in cancer. Oren, M. and V. Rotter. Cold Spring Harbor Perspectives in Biology. 2010 Feb;2(2):a001107...

  6. TALEN Plasmids and Kits

    Type
    Collection
    ...also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important ...

  7. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTCTGGGCTCGATCTTGAgctcgatggaaaaaccTCCAACCGTTCACGATCA spry4 TAL3182 & TAL3183 TCATGGTGCAGCCGTTACtggacagccgcgttccCTACGGACGCCTCCAGCA...

  8. Deisseroth INTRSECT Collection

    Type
    Collection
    ...producing their own INTRSECT plasmids. Online spreadsheet (Link opens in a new window) of Flp-recombinase-expressing...

  10. Plan Your Experiment

    Type
    Guide
    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on...transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to ...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic...

  11. Modular Cloning Guide

    Type
    Guide
    ...multiplexed CRISPR editing, and more. Fungal Toolkit for Modular Cloning (FTK) Fungal Expression, CRISPR Arnold...Bacterial Expression, CRISPR Veronica Godoy-Carter Plasmids for protein expression and CRISPRi experiments in...experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering, CRISPR, Mammalian Expression Takashi...MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9 Genome Engineering, CRISPR Sylvestre Marillonnet...SpCas9-coding gene. MoClo CRISPR/Cas Toolkit for Plants Genome Engineering, CRISPR, Plant Expression Vladimir...Engineering, CRISPR, Plant Expression Johannes Stuttmann Plasmids for assembly of constructs for CRISPR genome...Genome Engineering, CRISPR, Yeast Expression Brigitte Gasser Plasmids to perform CRISPR/Cas9 mediated genome...

  12. Guide to Using Pooled Libraries

    Type
    Guide
    ...Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Pooled CRISPR Libraries...protocol. Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our...of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells. DIVERGE... plasmids have been depleted by selection. In a CRISPR screen, negative screens are often used to identify...

  13. Addgene's eBook Collection

    Type
    Guide
    ...Close About Overlay Select CRISPR 101 January 2021 | Third Edition About CRISPR 101 January 2021 | ...Overlay Select CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español...experimental advice such as tips for planning your CRISPR experiment and designing your gRNA and how to use...traducción al español de nuestro libro electrónico, CRISPR 101. Close About Overlay Select Antibodies 101 ...

  14. Science Guides

    Type
    Guide
    ...Read More CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems...background information on CRISPR and the basics for planning your first CRISPR experiment. Read More Optogenetics...links to the guides below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies...

  15. Lentiviral Vector Guide

    Type
    Guide
    ...Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide screens are an efficient...Addgene has an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation...and base editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic...Popular uses include Cre-lox mice, knockouts using CRISPR or shRNA , and overexpressing of oncogenes and ...Doench, J. G., & Zhang, F. (2013). Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science...

  16. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... Addgene. CRISPR/Cas9 Delivery rAAV vectors are a popular choice for the delivery of CRISPR/Cas9 elements...size, low immunogenicity, and limited ability to spread after injection. Different AAV serotypes exhibit...making it one of the most common methods for in vivo CRISPR-based genome editing. Packaging the Cas9 from Streptococcus...blog post on overcoming AAV size limitation for CRISPR delivery for more information. Split AAVs to increase...

  17. Molecular Biology Reference

    Type
    Guide
    ...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and ...the genome. For more information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for...

  18. Educational Resources

    Type
    Guide
    ...to our collections. Titles include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 ...include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology Career Readiness...in-depth instructional content on subjects such as: CRISPR Chemogenetics Optogenetics Genetic Code Expansion...

  19. Chemogenetics Guide

    Type
    Guide
    ...different AAV serotypes have varying degrees of viral spread within injected tissue, and this must be considered...especially useful in AAV experiments where the virus can spread around the injection site and potentially express...

  20. Adenovirus Guide

    Type
    Guide
    ...of time or when needing to package larger cargo. CRISPR-Cas9 Delivery As compared to other viral vectors...Production and Troubleshooting Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells...
Showing: 541 - 560 of 562 results