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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 560 results for: mme

Showing: 541 - 560 of 560 results

  1. Fluorescence Titering Assay

    Type
    Protocol
    ...Titer will vary between cell lines. It is not recommended to subject lentiviral supernatants to multiple...you are freezing and aliquoting virus, it is recommended that you titer from the frozen stock to account...

  2. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... be done underneath a hood. Clean any spills immediately, and decontaminate as necessary. For large spills...bloodborne pathogens training. It is strongly recommended that anyone participating in BSL-2 work receives...

  3. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...digestion . Most restriction enzymes digest DNA asymmetrically across their recognition sequence, which results... 1 vector molar ratio will work just fine. We recommend around 100ng of total DNA in a standard ligation...

  4. Immunocytochemistry

    Type
    Protocol
    ...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...antibody concentrations, incubation times, and recommended compatible reagents. Secondary antibodies must...

  5. Colony Formation Titering Assay

    Type
    Protocol
    ...Titer will vary between cell lines. It is not recommended that lentiviral supernatants be subjected to ...you are freezing and aliquoting virus, it is recommended that you titer from the frozen stock to account...

  6. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...water IgG isotype standard 2.5 mg/mL - can use commercial standard or validate an in-house standard Before...

  7. Western Blot

    Type
    Protocol
    ...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...a clean microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section...

  8. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...wash, aspirate the wash buffer from the wells. Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide...

  10. Protocol - How to Streak a Plate

    Type
    Protocol
    ...setting is important for organization, and it is recommended that you keep a standard labeling system for ...

  13. Centrifugation

    Type
    Protocol
    ... unbalanced or improperly loaded centrifuge. Immediately stop the centrifuge and investigate the issue...

  20. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Pro-Tip Stirring during the cooling period is recommended or the solution may separate into phases. Aliquot...
Showing: 541 - 560 of 560 results