We narrowed to 565 results for: mme

...to use of your purified virus. Nonetheless, we recommend performing a buffer exchange before using the ...QuickSeal tube at the bottom using an 18 ga needle. Immediately start collecting 0.5 to 1 mL fractions in microcentrifuge...that has settled at the bottom of the column. We recommend concentrating to a minimum of 500 µL. If the concentrate...

...important to digest plenty of starting material. We recommend 1.5-2μg of donor plasmid and 1μg of recipient ...space to cut out the bands. Because of this we recommend that you use a wide gel comb, run the gel on the...fuse your insert to your recipient plasmid. We recommend around 100ng of total DNA in a standard ligation...

... reduce the risk of contaminating reagents we recommend making small aliquots of master mixes, primers...Buffer containing 0.05% Poloxamer 188: Prepare immediately before use and vortex before using. 500 µL of... > 10 to be invalid. To reduce NTC values, we recommend wiping down all pipettes and equipment with 10%...

...of each sample. Samples can be used for ddPCR immediately or stored at -20 °C until ready to use. Preparing...infectious titer. Tips and Troubleshooting We recommend wiping down all pipettes and equipment with 10%... reduce the risk of contaminating reagents we recommend making small aliquots of master mixes, and primers...

...given sequence. For a list of the commonly used commercially available restriction enzymes, see New England...aliquot of your plasmid of interest (see below for recommended amounts) Appropriate restriction enzyme (see ...

...Titer will vary between cell lines. It is not recommended to subject lentiviral supernatants to multiple...you are freezing and aliquoting virus, it is recommended that you titer from the frozen stock to account...

...antibiotic powder can be dissolved in dH 2 0. Addgene recommends making 1000X stock solutions and storing aliquots...Antibiotic Concentrations Commonly Used Antibiotics Recommended Concentration Ampicillin 100 µg/mL Bleocin 5 ...

... be done underneath a hood. Clean any spills immediately, and decontaminate as necessary. For large spills...bloodborne pathogens training. It is strongly recommended that anyone participating in BSL-2 work receives...

...digestion . Most restriction enzymes digest DNA asymmetrically across their recognition sequence, which results... 1 vector molar ratio will work just fine. We recommend around 100ng of total DNA in a standard ligation...

...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...antibody concentrations, incubation times, and recommended compatible reagents. Secondary antibodies must...

...Titer will vary between cell lines. It is not recommended that lentiviral supernatants be subjected to ...you are freezing and aliquoting virus, it is recommended that you titer from the frozen stock to account...

...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...water IgG isotype standard 2.5 mg/mL - can use commercial standard or validate an in-house standard Before...

...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...a clean microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section...

...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...wash, aspirate the wash buffer from the wells. Immediately before use, mix 5 mL TMB Solution and 5 mL Peroxide...

...100% glycerol in dH20. Snap top tubes are not recommended as they can open unexpectedly at -80°C. Freeze...

...setting is important for organization, and it is recommended that you keep a standard labeling system for ...

...from the gel. This is most commonly done with a commercial gel purification kit, such as the (Link opens...

... unbalanced or improperly loaded centrifuge. Immediately stop the centrifuge and investigate the issue...

...Kit Extract RNA from tissues or cells without a commercial kit Plasmid Cloning Protocols for constructing...

...between products. Addgene does not endorse or recommend specific products or equipment. Inclusion of this...