Hot Plasmids: Summer 2024
Type
Blog Post
...Conventional cryogenic electron microscopy (cryo-EM) protocols typically require high concentration and purity...
We narrowed to 660 results for: otos
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Antibodies 101: Beyond Surface Labeling
TypeBlog Post...non-surface markers, a separate intracellular labeling protocol is necessary, requiring chemical treatment of ... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013). PubMed. A Mouse ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post... sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end or paired-end... -
10 Steps to a Perfect Science Talk
TypeBlog Post... find it. Experienced speakers present useful protocols, geeky science details, products that resulted... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... if your research is dependent on a pathology microtome/cryostat, then making a careful decision on the... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...knockout mouse models using long ssDNA donors.” Nat Protoc. 13 (2018) (1):195-215. DOI: 10.1038/nprot.2017.153... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...AAV8, AAV9 Lung AAV6 Pancreas AAV2, AAV8 Photoreceptor cells AAV2, AAV5, AAV8 Skeletal muscle AAV1... -
Plasmids 101: Dimers and Multimers
TypeBlog Post... Biology ReferenceAddgene's Restriction Digest Protocol More resources on the Addgene blog: Plasmids ... -
Plasmids 101: Restriction Cloning
TypeBlog Post...for more detail. Find columnless purification protocols on our website. 6. Verify the plasmid After purifying... -
Finding Your Perfect Job After University
TypeBlog Post...science. Another problem was that the work became monotonous very quickly; as soon as I learned how to do ... -
CRISPR Plasmids - Xenopus
TypeCollection...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content... -
CRISPR Plasmids - Zebrafish
TypeCollection...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content... -
CRISPR Plasmids - C. elegans
TypeCollection...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content... -
Antibodies 101: Validation
TypeBlog Post...Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto T (2016) A proposal for validation of antibodies... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...D. (2000). Random mutagenesis by PCR. Current Protocols in Molecular Biology, 51(1). https://doi.org/10.1002... -
CRISPR Plasmids - Drosophila
TypeCollection...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content... -
CRISPR Plasmids - dCas9-FokI
TypeCollection...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically... -
CRISPR Plasmids - Repress Gene Expression
TypeCollection...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...