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  8. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...for more detail. Find columnless purification protocols on our website. 6. Verify the plasmid After purifying...

  11. Antibodies 101: Validation

    Type
    Blog Post
    ...Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto T (2016) A proposal for validation of antibodies...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires...accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering...

  14. Optogenetics Guide

    Type
    Guide
    ...reduces photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein... 400 UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with slow reversal...responses, such as nonspecific cation influx vs. proton efflux. Researchers have used genetic engineering...neuron. Feature enhancements include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...basic-hFX-promotor(1kb) FXN hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-promotor(1,3kb) FXN...basic-hFX-promotor(2,5kb) FXN hFXN Friedreich ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor(2kb, distal...pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH, Strep, His T7 ALS, motor neuron disease Bernd Bukau 169713 A1-LCD WT HNRNPA1...

  18. CRISPR Guide

    Type
    Guide
    ...) CRISPR software and depositor protocols JOVE video reprint: Protocol for genomic deletions in mammalian...target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...before use (Figure 8C), following the amplification protocol specified by the depositing lab. After amplification...The translated codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target...engineering using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A....

  19. Educational Resources

    Type
    Guide
    ...including protocols, tips on using Addgene’s materials, and career advice. Playlists include: Protocols Careers... Molecular Cloning Techniques Protocols Find written and video protocols for a wide range of applications...videos, and protocols Educational... Educational Resources From eBooks to videos and blog posts to protocols, our educational...

  20. Plan Your Experiment

    Type
    Guide
    ...Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic deletions...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...Biology . 1239 , 197–217. PMID: 25408407 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the...
Showing: 541 - 560 of 654 results