We narrowed to 580 results for: vit;

1. An Integrin Antibody Toolkit from IPI

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   Blog Post

   ...IPI antibodies  Antibody Target Proteins Reactivity Isotype Source Species Anti-Integrin alpha...

2. CRISPR 101: Homology Directed Repair

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   ...Easi-CRISPR (Quadros et al., 2017). Easi-CRISPR in vitro transcribes RNA encoding your repair template, then...

3. Evolution of Lab Techniques

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   ...synthesized by electronically recording fluorescence activity as fluorescently labeled nucleotides are incorporated...

4. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   ...assembly a ligase is added to repair the nicks in vitro, whereas in SLIC these constructs are repaired in...

5. Treating Muscular Dystrophy with CRISPR Gene Editing

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   ...effects, each group finds very low to no off-target activity at the ten highest-ranking predicted off-target...

6. How to Negotiate a Successful Lab Start Up Budget

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   ...opinions on creating and negotiating budgets and invite you to leave a comment below Happy Sciencing, ...

7. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   Blog Post

   ...proteins and confirmed that there was no cross-reactivity, opening the door for immunostaining analysis...

8. CRISPR 101: Off-Target Effects

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   ...by Cas does not always correlate with catalytic activity at those sites, especially when DNA mismatches...

9. Antibodies 101: Multiplex Immunofluorescence

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   ...handling steps, or there’s some unexpected cross-reactivity. Keep in mind that you may need to optimize the...

10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...regulation, as long as they can control recombinase activity. For example, post-transcriptional recombinase...

11. PiggyBac-ing Through the Genome Editing Field

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    ...repeats (ITRs) found on the piggyBac™ donor for its activity. In summary, the piggyBac™ transposon is a powerful...

12. Plasmids 101: Restriction Cloning

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    ...stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...

13. Finding Your Perfect Job After University

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    ...have meetings between teams. This communication is vital because otherwise I’d lose touch with my colleagues...

14. Building Global Connections with the International Mentorship Program USA-EUROPE

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    ... metastasis as well as teaching and mentoring activities. Follow her on twitter @zcastan or on linkedln...

15. Important Considerations When Using AAVs

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    ...virus serotypes: impact of larger genomes on infectivity and postentry steps." Journal of virology 79.15...

16. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...expressing TH protein indicating that there is promoter activity. Additionally, some neurons express low levels...

17. A Guide to Getting Started in Undergrad Research

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    ...When you are hunting for labs to join, one of the vital things to look for is a supportive environment—it...

18. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ...particularly interested in science communication and in vitro diagnostics.     References 1. Costantino N, Court...

19. Antibodies 101: Introduction to Antibodies

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    ...sequences can be optimized for specificity and sensitivity, for example by changing certain amino acids ...

20. With an Eye Towards the Future, We Look Back at the March for Science

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    Blog Post

    ... clinic regulations, evidence-based research is vital in combating misinformation and propaganda that ...