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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,076 results for: TOR;

Showing: 841 - 870 of 1076 results

  6. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids Recovering Plasmid DNA from Bacterial...temperature. Now screw on the top of the bottle and store the LB at room temperature. When ready to grow your...bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require growth... overnight incubation. Optional: For long term storage of the bacteria, you can proceed with Creating ...

  7. Video Library

    Type
    Protocol
    ... Protocols Video protocol guides for standard laboratory procedures. How-To Videos Screencapture walk-...enzymes Diagnostic Restriction Digest Protocol Storing and Handling Addgene Plasmids What to do after ...receive your plasmids from Addgene Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR...Electrophoresis Gel electrophoresis is the standard laboratory procedure for separating DNA by size for visualization...

  8. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  9. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Ready to start tagging? We are here to help with vectors, protocols, antibodies, and more!   Resources ...

  14. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...was originally derived from jellyfish (Aequorea victoria) and can serve as an epitope tag as well as a ...

  21. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...limitations of packaging size associated with viral vectors. For these reasons, iTOP, especially in tandem ...

  22. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... mM EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution ...acetate 23 mL glacial acetic acid 57 mL of dH 2 O Store Solution III at 4°C Grow 2 mL overnight cultures... room temperature before quantifying and using. Store DNA at 4°C. Tips and FAQ Plasmid purification kits...

  27. How to Lead a Great Meeting

    Type
    Blog Post
    ... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ...

  28. Components of CRISPR/Cas9

    Type
    Blog Post
    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...
Showing: 841 - 870 of 1076 results