We narrowed to 563 results for: cat.3

1. Pouring LB Agar Plates

   Type

   Protocol

   ...on plates without any antibiotic. Negative Result 3: Only the Non-resistant Strain Grows If only the non-resistant...Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated, the antibiotic powder can be dissolved in dH ...test results. Sample Data In all cases below (-) indicates that the tested strain is not supposed to be resistant... resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the ...

2. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...stable cell pool were then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western...without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment of adherent cells) Trypsin...orange/yellow if using a standard phenol red pH indicator) because the buildup of waste products could be...

3. Antibody Guide

   Type

   Guide

   ... a protein’s presence or absence. Figure 3: A western blot detecting proteins using the indirect detection...Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods Visualization...Antibody Applications Antibody-based applications can be generally classed into four categories: Quantification... Read on to learn more about the applications common to each category, including an outline of the basic...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...concentrates in the sample. Controls for quantification methods Quantification methods should be run with: a positive...using sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process...