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TypeBlog Post...simple question is a moving target. There are a multitude of options for achieving success in a science ...
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Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...much as possible. However, when you need to label multiple markers, some bleeding is often unavoidable. But... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...spectral analysis of fluorescent proteins for multiphoton microscopy." Journal of biomedical optics 17.9... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post.../Church (7). These reference libraries contain multiple gRNAs targeting all genes in the indicated species... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...tens of thousands of biomolecules each containing multiple binding crevices that can nonspecifically soak... -
Selecting Your Plasmid Purification Kit
TypeBlog Post... Multiple Authors... -
CRISPR Guide
TypeGuide...edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter...leveraged to achieve specific goals, such as targeting multiple genomic locations or enhancing specificity, efficiency...experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all ...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell... -
Adenovirus Guide
TypeGuide...genomes range between 26–45 kb in length and contain multiple heavily-spliced transcripts flanked by two inverted...harvested about 7–10 days later. Vogelstein designed multiple shuttle plasmids for different purposes. The pAdTrack...that the infection rates are equivalent across multiple viruses. The adenoviral backbone plasmid pAdEasy... also been developed as vaccine candidates for multiple infectious diseases and prostate cancer. Cancer... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within... -
Lentiviral Vector Guide
TypeGuide...integration sites have led to the development of multiple genome-wide analysis pipelines to assess viral...in the envelope and packaging plasmids. Unless multiple recombination events occur between the packaging.... (2021). CReVIS-Seq: A highly accurate and multiplexable method for genome-wide mapping of lentiviral...Mandel, R. J., Naldini, L., & Trono, D. (1997). Multiply attenuated lentiviral vector achieves efficient... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within... -
Guide to Using Pooled Libraries
TypeGuide... unique gene targeting sequence, but there are multiple sequences targeting each gene in the overall library...lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen can help...library screens, cells are infected at a very low multiplicity of infection (MOI) to increase the odds that... -
Adeno-associated virus (AAV) Guide
TypeGuide...Hybrid capsids are engineered capsids derived from multiple different serotypes, which can further alter transduction... the CNS. Concatemer A DNA segment composed of multiple, repeated DNA sequences linked end-to-end. Episome... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within... -
Cloning
TypeGuide... same sites in the same orientation within its multiple cloning site . Due to their short length, it is...cloning is that it allows for the simple assembly of multiple fragments of DNA in the chosen orientation, and... -
Optogenetics Guide
TypeGuide...activation wavelengths also make it possible to combine multiple opsins in the same experiment. For example, you...196:1-28. PMID 20303157 Han X, Boyden ES. 2007 Multiple-color optical activation, silencing, and desynchronization... -
Gamma-Retroviral Vector Guide
TypeGuide...in the envelope and packaging plasmids. Unless multiple recombination events occur between the packaging... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within... -
Chemogenetics Guide
TypeGuide... et al. (2015). A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior. Neuron...