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We narrowed to 578 results for: multi

Showing: 561 - 578 of 578 results

  1. Finding Your Science Policy Path

    Type
    Blog Post
    ...simple question is a moving target. There are a multitude of options for achieving success in a science ...

  8. Antibody Guide

    Type
    Guide
    ...five human isotypes. Some isotypes naturally form multi-antibody units, while others exist as monomers. ...antibody in assays with multiple targets, and is described in more detail in the Multiplex Assays section below...differentiation between multiple antibodies. They are often used in assays that have multiple proteins to detect... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...allows for the amplification of signals by having multiple secondary antibodies binding to a primary antibody..., which is typically the number of antibodies multiplied by two, as each antibody has two binding sites...

  9. CRISPR Guide

    Type
    Guide
    ...edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter...leveraged to achieve specific goals, such as targeting multiple genomic locations or enhancing specificity, efficiency...Figure 2: Overview of the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve...more than one gene. In these cases, delivering multiple gRNAs using a single plasmid ensures that all ...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...

  10. Adenovirus Guide

    Type
    Guide
    ...genomes range between 26–45 kb in length and contain multiple heavily-spliced transcripts flanked by two inverted...harvested about 7–10 days later. Vogelstein designed multiple shuttle plasmids for different purposes. The pAdTrack...that the infection rates are equivalent across multiple viruses. The adenoviral backbone plasmid pAdEasy... also been developed as vaccine candidates for multiple infectious diseases and prostate cancer. Cancer... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...integration sites have led to the development of multiple genome-wide analysis pipelines to assess viral...in the envelope and packaging plasmids. Unless multiple recombination events occur between the packaging.... (2021). CReVIS-Seq: A highly accurate and multiplexable method for genome-wide mapping of lentiviral...Mandel, R. J., Naldini, L., & Trono, D. (1997). Multiply attenuated lentiviral vector achieves efficient... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

  12. Guide to Using Pooled Libraries

    Type
    Guide
    ... unique gene targeting sequence, but there are multiple sequences targeting each gene in the overall library...lineage tracing or parsing the effects of expressing multiple genes at once. A well-designed screen can help...library screens, cells are infected at a very low multiplicity of infection (MOI) to increase the odds that...

  13. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Hybrid capsids are engineered capsids derived from multiple different serotypes, which can further alter transduction... the CNS. Concatemer A DNA segment composed of multiple, repeated DNA sequences linked end-to-end. Episome... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

  14. Molecular Cloning Techniques

    Type
    Guide
    ...popular restriction enzyme sites, usually within the multiple cloning site (MCS). You can easily move (subclone... cloning is that it allows for the assembly of multiple fragments of DNA in the chosen orientation at ...

  15. Optogenetics Guide

    Type
    Guide
    ...activation wavelengths also make it possible to combine multiple opsins in the same experiment. For example, you...196:1-28. PMID 20303157 Han X, Boyden ES. 2007 Multiple-color optical activation, silencing, and desynchronization...

  16. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...in the envelope and packaging plasmids. Unless multiple recombination events occur between the packaging... Infection The natural process of entry and multiplication of a pathogen, like viruses or bacteria, within...

  17. Promoters

    Type
    Guide
    ...transcription elongation factor promoter TRE Inducible Multiple Tetracycline response element promoter; often ...

  18. Chemogenetics Guide

    Type
    Guide
    ... et al. (2015). A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior. Neuron...
Showing: 561 - 578 of 578 results