We narrowed to 602 results for: she

1. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   ...Cassel SH, Vazquez F, Weir BA, Hahn WC, Marsh JA, Tsherniak A, Kadoch C (2018) Interrogation of Mammalian ...

2. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   ...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...

3. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

4. Lentivirus Production

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   Protocol

   ...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

5. Harnessing Bacterial Toxins for Allelic Exchange

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   Blog Post

   ... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...

6. Hot Plasmids: Summer 2024

   Type

   Blog Post

   ..., particle orientation distribution and Fourier-shell correlation curve (D), and the reconstructed 3D ...

7. Five Popular Model Organisms, Part 2

   Type

   Blog Post

   ...with their regenerative abilities is expected to shed light on human aging pathological developments (...

8. Transferable Skills Guide: Conflict Resolution

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   Blog Post

   ...want, but that means the other party will feel punished or invalidated. Both compromise and arbitration...

9. Antibodies 101: Introduction to Immunofluorescence

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   Blog Post

   ...reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique ...

10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    ... Alyssa Shepard...

11. CRISPR 101: Homology Directed Repair

    Type

    Blog Post

    ...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

12. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    Blog Post

    ...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

13. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

14. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

    ...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

15. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post

    ... GeneArt® Seamless Cloning and Assembly (Thermo Fisher Scientific) NEBuilder® HiFi DNA Assembly (NEB) ...

16. AAV Purification by Iodixanol Gradient Ultracentrifugation

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    Protocol

    ...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian...

17. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ... which will disrupt the gel. Any bubbles can be pushed away from the well comb or towards the sides/edges...

18. Handling Plasmids from Addgene - Purifying Plasmid DNA

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    Protocol

    ...vortex at this stage or the genomic DNA will become sheared and will therefore contaminate your purified plasmid...

19. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

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    Protocol

    ...to insert in the ligation reaction. Isolate the Finished Plasmid Finally, you will need to pick individual...

20. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...