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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 405 results for: pho;

Showing: 21 - 30 of 405 results

  1. Bacterial Expression Systems

    Type
    Collection
    ...collection of FPs, including photoactivatable, photoconvertible, and photoswitchable proteins, organized by ...when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they are ...Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers...fluorescence both from the donor and the acceptor fluorophore by only exciting the donor. In BiFC, proteins...Inducible Promoters Repressible Promoters Synthetic Photobiology: Optogenetics for E. coli Return to top Reporter...

  2. Cre-lox system

    Type
    Collection
    ...pAAV-CIBN-CreC photo-activatable cre recombinase CAG AAV Bruchas 75268 pAAV-CRY2-CreN photo-activatable ... Cre and gRNAs cTnT AAV Pu 87694 pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2 light-activated Cre cTnT Mammalian Campbell...Cepko 13780 pNrl-Cre Cre-Myc, Expressed in rod photorecetor cells in the retina Nrl Mammalian Cepko 13962...pAAV-Syn-iCre iCre AAV Ritola 122960 pcDNA3.1_PA-Cre photo-activatable Cre recombinase CMV Mammalian Sato 122961...pBbE5a-Opto-Cre-Vvd Cre recombinase split with Vivid photodimers lacUV5 Bacterial Dunlop 135217 pDEST mfap4:icre-p2a-tomato...pAAV-EF1a-NCreV N-terminal Cre component split with Vivid photodimers EF-1 alpha AAV Zeng 140132 pAAV-EF1a-CCreV C-...C-terminal Cre component split with Vivid photodimers EF-1 alpha AAV Zeng 140135 pAAV-EF1a-iCreV Split...

  3. Recombinases AAV Preps

    Type
    Collection
    ...these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI....AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA...Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA...Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none...none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a ...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ... of photons. Its robust signal, sensitivity, wide dynamic range, and resistance to photobleaching have...Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and... from the luciferase reaction to an acceptor fluorophore can provide more flexibility in emission colors...BRET energy donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc... sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...to enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence...

  6. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Near-Infrared Long Stokes Shift Photoactivatable Photoconvertible Photoswitchable Timers Blue/UV Protein Excitation...LSSmKate2-C1 - Mammalian Expression Jump to Top Photoactivatable (e.g. off to on) Excitation and Emission wavelengths...HisB-PAiRFP2 - Bacterial Expression Jump to Top Photoconvertible (e.g. green to red) Protein Excitation (nm...pBAD-PSmOrange2 - Bacterial Expression Jump to Top Photoswitchable (e.g. off to on to off) Protein Excitation ...

  7. mTOR Pathway

    Type
    Collection
    ...PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6 Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory...AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD...Protor Also known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent companion...

  8. p53 Pathway

    Type
    Collection
    ...deprivation. p53 is activated through both phosphorylation and acetylation, which disrupt its interaction...ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also... 1 (RCHY1); E3 ubiquitin protein ligase PTEN Phosphatase and tensin homolog PUMA BCL2 binding component...

  9. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...2.1 may be omitted. CRISPR Cloning Anneal and phosphorylate oligos. Resuspend oligos at a concentration ...Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos may be ordered instead. For this approach...

  10. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...followed by a quick ligation of annealed and phosphorylated oligonucleotides containing the guide target...Prdm1 and Vsx2 enhancers in the retina alters photoreceptor and bipolar cell fate specification, yet differs...
Showing: 21 - 30 of 405 results